Reports of Biochemistry and Molecular Biology
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Basic Sciences
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2322-3480
2322-3480
10.61186/rbmb
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jalali
1398
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gregorian
2019
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The Protective Role of Gallic Acid Pretreatment On Renal Ischemia-reperfusion Injury in Rats
بیوشیمی
Biochemistry
مقالات اصلی
Original Article
<div style="text-align: justify;"><strong><em>Background:</em></strong> Renal ischemia-reperfusion injury (RIR) occurs when there is a temporary restriction of blood flow to the kidneys followed by an influx of blood, re-oxygenating the tissues. This occurs as a severe complication of major surgery. This process causes significant damage to the tissues and is responsible for the development of acute kidney injury (AKI), a life-threatening condition with high mortality rates. Here, we evaluated the potential protective effects of the antioxidant, gallic acid (GA), on RIR in an <em>in vivo</em> rat model.<br>
<br>
<strong><em>Methods:</em></strong> Adult male Sprague Dawley rats were randomly divided into three groups: group 1 (control, n = 8), group 2 (Ischemia-reperfusion (IR) with no-treatment, n = 7), and group 3 (IR + daily GA 100 mg/kg i.p, n = 7). The abdomens of the rats in the control group were opened during the surgical procedure, then sutured closed. GA pretreatment began daily 15 days prior to inducing RIR. To induce RIR, the umbilical arteries were obstructed on both sides and clamped with mild pressure for 45 min. Following the 45 min ischemia, the clamps were removed to allow for the induction of reperfusion. The reperfusion phase was 24 hours.<br>
<br>
<strong><em>Results:</em></strong> Following IR, the serum levels of urea and creatinine significantly increased compared to the controls. Pretreatment with GA was observed to reduce urea and creatinine levels following IR. However, this decrease was not statistically significant. The serum and renal levels of malondialdehyde (MDA) in the IR group was significantly elevated compared to the control group. Conversely, glutathione (GSH) levels and the activity of glutathione peroxidase (GPX) significantly decreased in the IR group compared to controls. Our findings show GA pretreatment to significantly improve the levels of renal MDA, serum GSH, and GPX activity following RIR.<br>
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<strong><em>Conclusions:</em></strong> Our findings highlight the protective role for GA in mitigating the damage caused by RIR and its applications as a potential treatment.<br>
</div>
Antioxidant enzymes, Gallic acid, Renal functional markers, Renal ischemia-reperfusion.
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48
http://rbmb.net/browse.php?a_code=A-10-66-3&slc_lang=en&sid=1
Hassan
Ahmadvand
hassan_a46@yahoo.com
10031947532846006545
10031947532846006545
No
Razi Herbal Medicine Research Center, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran & Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Banafsheh
Yalameha
negar.noury@gmail.com
10031947532846006546
10031947532846006546
No
Glavizh
Adibhesami
glavizh.adib@gmail.com
10031947532846006547
10031947532846006547
No
Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Maryam
Nasri
maryam.nasri95@gmail.com
10031947532846006548
10031947532846006548
No
Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Negar
Naderi
naderinegar@yahoo.com
10031947532846006549
10031947532846006549
No
Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran.
Esmaeel
Babaeenezhad
es.babaeenezhad1391@gmail.com
10031947532846006550
10031947532846006550
No
Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Negar
Nouryazdan
negar.noury@gmail.com
10031947532846006551
10031947532846006551
Yes
Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.