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en Frequency of Listeria monocytogenes Isolated from Diarrhea Samples of Pediatric Patients at Central Iran میکروب شناسی Microbiology مقالات اصلی Original Article <div style="text-align: justify;">Background:  Listeria monocytogenes is a primarily foodborne bacterial pathogen that is one of the causative agents of gastroenteritis. However, the prevalence of L. monocytogenes infection in pediatric patients with diarrheal disease is not clearly identified in the Iranian population. This study aimed to investigate the frequency of L. monocytogenes isolates found in infectious diarrhea samples of pediatric patients in an Iranian population. Methods: A total of 173 infectious diarrhea samples collected from pediatric patients were used in this cross-sectional study. Samples were collected from patients referred to the Children’s Educational-Therapeutic Center affiliated with the Arak University of Medical Sciences in Arak, Iran from May-September 2015. To identify the presence of L. monocytogenes, the samples were directly inoculated into the Listeria Enrichment Broth Base through cold enrichment, then plated onto isolated exclusive Listeria Selective Agar Base. As an alternative method for identifying L, monocytogenes, Polymerase Chain Reaction (PCR) of the InlA gene was used. Results:  Of the 173 infectious diarrhea samples, eight (4.6%) with L. monocytogenes were identified using exclusive culture media, while nine (5.2%) were identified using PCR. The majority of L. monocytogenes infections (seven cases (77.7%)) were observed in children under the age of five. Conclusions: Our results show L. monocytogenes infections to have a low prevalence for causing diarrhea in children in the central region of Iran. This should be taken into consideration by pediatricians when treating intestinal diseases.</div> Diarrhea, Iran, Listeria monocytogenes, Pediatric. 172 177 http://rbmb.net/browse.php?a_code=A-10-271-1&slc_lang=en&sid=1 Elnaz Abbasi elnaz.abbasi@yahoo.com 100319475328460017277 100319475328460017277 No Department of Microbiology & Immunology, Faculty of Medicine, Arak University of Medical Sciences. & Department of Microbiology & Immunology, Khomein University of Medical Sciences, Khomein, Iran. Alireza Amouzandeh-Nobaveh aamouzandeh@yahoo.com 100319475328460017278 100319475328460017278 No Department of Microbiology & Immunology, Faculty of Medicine, Arak University of Medical Sciences. Ehsanollah Ghaznavi-Rad ghaznaviehs@yahoo.com 100319475328460017279 100319475328460017279 Yes Department of Microbiology & Immunology, Faculty of Medicine, Arak University of Medical Sciences. & Molecular and Medicine Research Center, Faculty of Medicine Arak University of Medical Sciences, Arak, Iran.