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en Association of Two Methylenetetrahydrofolate Reductase Polymorphisms (rs1801133, rs1801131) with the Risk of Type 2 Diabetes in South-East of Iran زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: DNA methylation has been linked to the development and progression of multiple disorders including T2D. One significant enzyme involved in DNA methylation is methylene tetrahydrofolate reductase (MTHFR). This study was designed to evaluate the association between rs1801133 and rs1801131 polymorphisms, located in the MTHFR, and T2D in an Iranian population. Methods: Blood samples from 151 patients with T2D and 136 healthy individuals were collected and DNA was extracted using the salting out method. Variants were genotyped using amplification tetra-refractory mutation system-polymerase chain reaction analysis. The data were analyzed via independent sample t-test and x2 tests. Results: The rs1801131 A/C polymorphism significantly increased the risk of T2D in codominant heterozygous AC (P=0.008), homozygous CC (P=0.01), and recessive CC (P=0.001) genotypes. Significant correlations were found regarding rs1801133 T/C gene polymorphism and the risk of T2D in codominant heterozygous TC (P=0.001), homozygote CC (P=0.001), and recessive CC (P=0.0001) models. The presence of the C allele is a potential risk factor for T2D for rs1801133 T/C (P=0.001) and rs1801131 A/C (P=0.04) polymorphisms. Conclusions: Both the rs1801133 T/C and rs1801131 A/C MTHFR polymorphisms significantly increased the risk of T2D in our population. Further studies in other ethnicities are necessary to verify our findings.  </div> Gene Polymorphism, MTHFR, Type 2 diabetes. 178 183 http://rbmb.net/browse.php?a_code=A-10-53-3&slc_lang=en&sid=1 Maryam Poodineh mpoodineh57@gmail.com 100319475328460017280 100319475328460017280 No Department of Biology, Payame Noor University of Taft, Yazd, Iran. Ramin Saravani saravaniramin@yahoo.com 100319475328460017281 100319475328460017281 Yes Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran. & Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran. Mahboubeh Mirhosseini m.mirhossain@gmail.com 100319475328460017282 100319475328460017282 No Department of Biology, Payame Noor University of Taft, Yazd, Iran. Saman Sargazi sgz.biomed@gmail.com 100319475328460017283 100319475328460017283 No Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.