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'); Reports of Biochemistry and Molecular Biology rbmb.net Basic Sciences http://rbmb.net 1 admin 2322-3480 2322-3480 10.61882/rbmb en jalali 1392 2 1 gregorian 2013 5 1 1 2 online 1 fulltext
en The Role of Anti-CCD Antibodies in Grape Allergy Diagnosis ایمنی شناسی Immunology <p style="text-align: justify;"><strong><em>Background: </em></strong>Allergens are mostly composed of glycoprotein structures. It is believed that glycan-specific antibodies may lead to false-positive reactions in immunoassays. In this study we investigated the glycosylation state of grape allergens as well as the presence of antibodies to cross-reactive carbohydrate determinants (anti-CCDs) in sera from grape-sensitive individuals.</p> <p style="text-align: justify;"><em><strong>Methods:</strong></em> Grape extract proteins were electrotransferred onto PVDF membranes and their glycosylation states were analyzed by blotting methods. To assess the presence of anti-CCDs, natural and mildly deglycosylated proteins were immunoblotted with grape-allergic subjects&#39; sera. We also measured the IgE reactivity of each subject&rsquo;s sera with other fruit extracts via an indirect ELISA.</p> <p style="text-align: justify;"><em><strong>Results:</strong></em> Immunoblotting studies showed that mildly deglycosylated grape proteins had lower IgE-binding capacity than their intact natural counterparts, which could be due to the presence of anti-CCDs. Biotinylation studies confirmed that the glycosylation levels of the 24, 32, and 60 kDa IgE-reactive proteins were higher than those of the 38 and 45 kDa ones. Lectin blotting showed that the 24 and 60 kDa bands were highly mannosylated, with the highest level of mannosylation on the 24 kDa allergen.</p> <p style="text-align: justify;"><em><strong>Conclusion: </strong></em>This study showed that some grape allergens are glycosylated and that anti-CCD antibodies may cause weakly false-positive results during assessment of IgE reactivity to grape allergens.</p> Allergy, Antibody, Cross-reactive carbohydrate determinants, Grape 74 81 http://rbmb.net/browse.php?a_code=A-10-1-12&slc_lang=en&sid=1 Reza Falak 10031947532846005933 10031947532846005933 No Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Mojtaba Sankian 10031947532846005934 10031947532846005934 No Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Hanieh Ketabdar 10031947532846005935 10031947532846005935 No Department of Biology, Faculty of Science, Islamic Azad University, Mashhad Branch, Mashhad, Iran Malihe Moghadam 10031947532846005936 10031947532846005936 No Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Abdol-Reza Varasteh varasteha@mums.ac.ir 10031947532846005937 10031947532846005937 Yes Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran