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en Dysregulated KDR and FLT1 Gene Expression in Colorectal Cancer Patients زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: Colorectal cancer (CRC) is one of the most commonly-diagnosed malignancies throughout the world and the fourth-leading cause of cancer deaths globally. Angiogenesis and the resultant tumor neovascularization is a well-known cancer hallmark. Here we investigated the expression of FLT1 and KDR, the influential genes in angiogenesis regulation, in CRC patients. Methods: We assessed FLT1 and KDR mRNA expression in 47 CRC samples and matched adjacent non-cancerous tissues (ANCT) by quantitative real-time PCR. The Spearmen correlation coefficient and receiver operating characteristic (ROC) curves were also examined. Results: Both genes were expressed at significantly greater levels in CRC tissues than in ANCT (p < 0.05). A significant association was found between KDR expression and disease stage and lymph status in CRC patients. Furthermore, the Spearman correlation demonstrated a moderate correlation between FLT1 and KDR expression in CRC samples. Finally, ROC curve analysis demonstrated that FLT1 had the greatest sensitivity (85.1%), while the greatest specificity was achieved by a combination of the two genes. Conclusions: The dysregulated FLT1 and KDR expression, in addition to the observed correlation and ROC curve results, indicate the critical importance of angiogenesis among the cancer pathways in CRC. These data can broaden our current knowledge of angiogenesis in CRC to improve disease diagnosis and patient treatment.  </div> Colorectal cancer, FLT1, KDR, Gene expression. 244 252 http://rbmb.net/browse.php?a_code=A-10-187-4&slc_lang=en&sid=1 Fatemeh Mohammad Rezaei f_m.rezaei@yahoo.com 100319475328460017340 100319475328460017340 No Department of Medical Genetic, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. & Tuberculosis and Lung Disease Research Center of Tabriz University of Medical Sciences, Tabriz, Iran. Shahryar Hashemzadeh shahriar_90@yahoo.com 100319475328460017341 100319475328460017341 No Tuberculosis and Lung Disease Research Center of Tabriz University of Medical Sciences, Tabriz, Iran. & General and Vascular Surgery Department of Tabriz University of Medical Sciences, Tabriz, Iran. Reyhaneh Ravanbakhsh Gavgani r.ravanbakhsh.g@gmail.com 100319475328460017342 100319475328460017342 No Department of biological science, school of natural science, University of Tabriz, Tabriz, Iran. Mohammadali Hosseinpour Feizi pourfeizi@easp.ir 100319475328460017343 100319475328460017343 No Department of Animal Biology, University of Tabriz, Tabriz, Iran. Nasser Pouladi pooladi@yahoo.com 100319475328460017344 100319475328460017344 No Department of Biology, Azarbaijan Shahid Madani University, Tabriz, Iran. Hossein Samadi Kafil Kafilhs@tbzmed.ac.ir 100319475328460017345 100319475328460017345 No Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Leila Rostamizadeh rostami@yahoo.com 100319475328460017346 100319475328460017346 No Department of Medical Genetic, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Vahid Kholghi Oskooei vahidkholghioskooei@gmail.com 100319475328460017347 100319475328460017347 No Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Mohammad Taheri mohammad_823@yahoo.com 100319475328460017348 100319475328460017348 No Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Ebrahim Sakhinia esakhinia@yahoo.co.uk 100319475328460017349 100319475328460017349 Yes Tabriz Genetic Analysis Center (TGAC), Tabriz University of Medical Sciences, Tabriz, Iran.