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en Construction of an Expression Plasmid (Vector) Encoding Brucella melitensis Outer Membrane Protein, a Candidate for DNA Vaccine زیست شناسی ملکولی Molecular Biology Background: DNA vaccination with plasmid encoding bacterial, viral, and parasitic immunogens has been shown to be an attractive method to induce efficient immune responses. Bacteria of the genus Brucella are facultative intracellular pathogens for which new and efficient vaccines are needed. Methods: To evaluate the use of a DNA immunization strategy for protection against brucellosis, a plasmid containing the DNA encoding the Brucella melitensis (B. melitensis) 31 kDa outer membrane protein, as a potent immunogenic target, was constructed. Results: The constructed plasmid, pcDNA3.1+omp31, was injected intramuscularly into mice and the expression of omp31 RNA was assessed by RT-PCR. The integrity of the pcDNA3.1+omp31 construct was confirmed with restriction analysis and sequencing. Omp31 mRNA expression was verified by RT-PCR. Conclusion: Our results indicate that the pcDNA3.1+omp31 eukaryotic expression vector expresses omp31 mRNA and could be useful as a vaccine candidate. Brucella melitensis, DNA Vaccine, Omp31, PcDNA3.1 82 86 http://rbmb.net/browse.php?a_code=A-10-1-13&slc_lang=en&sid=1 Fatemeh Vahedi f.vahedi@rvsri.ac.ir 10031947532846005938 10031947532846005938 Yes Razi Vaccine and Serum Research Institute, Mashhad, Iran Elnaz Ghorbani 10031947532846005939 10031947532846005939 No Microbiology Department, Al-Zahra University, Tehran, Iran Tahereh Falsafi 10031947532846005940 10031947532846005940 No Microbiology Department, Al-Zahra University, Tehran, Iran