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en Achillea Wilhelmsii C. KochHydroalcoholic Extract Induces Apoptosis and Alters LIN28B and p53 Gene Expression in Hela Cervical Cancer Cells زیست شناسی سلولی Cell Biology مقالات اصلی Original Article <div style="text-align: justify;">Background:Inappropriate activation of the proto-oncogene LIN28Band inactivation of the p53 tumor suppressor, have been shown to have a critical role in tumorigenesis. Previousresearch has shown therapeutic potential for the use of herbal plants as an alternative strategyforcancer treatment. Achillae wilhelmsii C. Kochis a plant that has been traditionally used for its medicinal properties. The aim of this study was to investigate the cytotoxic and apoptosis-inducing effect of Achillea wilhelmsiiC. Kochhydroalcoholicextract (AWHE) on HeLa cervical cancer cells and its effect on LIN28Band p53 expression. Methods:The cytotoxic activity of AWHEwas evaluated on HeLa cells using a trypan blue exclusion assay. The Annexin V/PI double staining assay was used to evaluate the apoptosis-inducing effect of the extract. The expressionof LIN28Band p53mRNA was measured using the real-time-PCRmethod. Results:Treatment with AWHEwas shown to induce cytotoxicity in both time and concentration-dependent manners(P<0.05). The propositionof HeLa cells undergoing apoptosis increased with increasing concentrations of AWHE(P<0.05). The mRNA levels of p53increased following 12, 24, and 48 hours of AWHEtreatmentwhereasthe mRNA levels of LIN28Bwere significantly decreased after4 to 12 hours of AWHEtreatment (p<0.05). Conclusions:Our findings confirmed the pro-apoptotic function of AWHEon the cervical cancer HeLa cell line. This indicates that targeting the LIN28Bsignaling cascade may be a promising therapeutic strategy for cervical cancer. Further research is required to understand the therapeutic effects of AWHEin primary human cervical cancer cells and a pre-clinicalcervical cancer model.</div> Achillea, Apoptosis, Cervical cancer, LIN28B, p53 318 325 http://rbmb.net/browse.php?a_code=A-10-53-4&slc_lang=en&sid=1 Mehdi Rezai rezaim212@gmail.com 100319475328460017375 100319475328460017375 No Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan,Iran Ramin Saravani saravaniramin@yahoo,com 100319475328460017376 100319475328460017376 Yes Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan,Iran & Cellular and Molecular Research Center,Resistant Tuberculosis Institute,Zahedan University of Medical Sciences, Zahedan, Iran. Saman Sargazi sgz.biomed@gmail.com 100319475328460017377 100319475328460017377 No Cellular and Molecular Research Center,Resistant Tuberculosis Institute,Zahedan University of Medical Sciences, Zahedan, Iran. Mahdiyeh Moudi mahdiyehmodi@yahoo.com 100319475328460017378 100319475328460017378 No Genetics of Non-Communicable Disease Research Center, Zahedan University of Medical Sciences, Zahedan, Iran. Mahdieh Jafari Shahroudi mahdiehjafari6@gmail.com 100319475328460017379 100319475328460017379 No Cellular and Molecular Research Center,Resistant Tuberculosis Institute,Zahedan University of Medical Sciences, Zahedan, Iran. Roya Saravani roya.saravani@gmail.com 100319475328460017380 100319475328460017380 No Department of Chemistry, Iran University of Science and Technology; Tehran, Iran.