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Reports of Biochemistry and Molecular Biology
rbmb.net
Basic Sciences
http://rbmb.net
1
admin
2322-3480
2322-3480
10.61882/rbmb
en
jalali
1398
10
1
gregorian
2020
1
1
8
4
online
1
fulltext
en
Investigation of the Effects of Apple Trees Infection by Erwinia amylovora on the Expression of Pathogenesis-Related Proteins Homologous to Allergens
ایمنی شناسی
Immunology
مقالات اصلی
Original Article
<div style="text-align: justify;"><strong><em>Background:</em></strong> Pathogenesis-related (PR) proteins are induced in response to biotic and abiotic stresses. Some plant proteins, including Mal d 1, Mal d 2, and Mal d 3 in apple, are allergens. In this study, the effects of <em>Erwinia amylovora</em> infection of two apple cultivars, Red and Golden Delicious, on the expression of PR proteins homologous to Mal d 1, 2, and 3 were investigated.<br>
<br>
<strong><em>Methods:</em></strong> In natural conditions trees with or without disease symptoms were sampled. In addition, seeds of the cultivars were grown in a greenhouse and seedlings were examined in three groups: 1) those inoculated by <em>E. amylovora</em>, 2) those inoculated by sterilized distilled water, and 3) uninoculated. Real-time PCR was used to determine expression of the Mal d 1, 2, and 3 genes (<em>Mal d 1, 2, </em>and <em>3</em>) in infected and uninfected samples. Statistical analyses were performed using SPSS and graphs were produced by Excel. P values < 0.05 were considered significant.<br>
<br>
<strong><em>Results:</em></strong> The analysis of variance showed that in natural conditions the effect of infection on the mean relative expression of <em>Mal d 2 </em>and<em> 3</em> was significant, and more so in Red than in Golden Delicious. The analysis of variance of the greenhouse samples showed that the effect of infection on the mean relative expression of <em>Mal d 1, 2, </em>and<em> 3</em> in both cultivars was significant.<br>
<br>
<strong><em>Conclusions:</em></strong>Our results suggest that Mal d 2 is more related to plant defense than Mal d 1 or Mal d 3, and is more highly expressed in<em> E. amylovora</em>-resistant than in <em>E. amylovora</em>-sensitive cultivars.</div>
Allergens, Erwinia amylovora, Homologous, Pathogenesis-related proteins.
376
382
http://rbmb.net/browse.php?a_code=A-10-277-1&slc_lang=en&sid=1
Nasser
Beikzadeh
beiczadeh@gmail.com
100319475328460017413
100319475328460017413
Yes
Khorasan Razavi Agricultural and Natural Resources Research and Education Center, AREEO, Mashhad, Iran.
Abdol-Reza
Varasteh
varasteha@varastegan.ac.ir
100319475328460017414
100319475328460017414
No
Varastegan Institute for Medical Sciences, Mashhad, Iran.