Reports of Biochemistry and Molecular Biology
rbmb.net
Basic Sciences
http://rbmb.net
1
admin
2322-3480
2322-3480
10.61186/rbmb
en
jalali
1398
7
1
gregorian
2019
10
1
8
3
online
1
fulltext
en
Clinical Correlation of miR-200c/141 Cluster DNA Methylation and miR-141 Expression with the Clinicopathological Features of Colorectal Primary Lesions/Tumors
زیست شناسی ملکولی
Molecular Biology
مقالات اصلی
Original Article
<div style="text-align: justify;"><strong><em>Background:</em></strong> Abnormal DNA methylation leading to altered transcription of certain genes occurs frequently in colorectal cancer (CRC). As with protein-coding genes, microRNAs (miRNAs) may be targeted for methylation in CRC; however, the methylation state of miRNA genes in CRC, especially in primary lesions, has not yet been completely elucidated. To understand the impact of DNA methylation on the miR-200c/141 cluster promoter, we investigated the methylation and expression of miR-141 in precancerous lesions and colorectal cancer.<br>
<br>
<strong><em>Methods:</em></strong> In this cross-sectional study, 208 colorectal tissue samples, including 34 tumor tissue samples, 60 precancerous lesions with matched normal adjacent tissues, and 20 normal tissue samples, were collected. Promoter methylation of the miR-200c/141 cluster was studied using methylation-specific PCR. MiR-141 expression was examined using quantitative real-time PCR.<br>
<br>
<strong><em>Results:</em></strong> Our findings showed that the miR-200c/141 cluster promoter region was most frequently hypermethylated in colorectal tumors and adenomatous polyps, but unmethylated in hyperplastic polyp tissues (P < 0.001). DNA methylation of the miR-200c/141 cluster and the tumor stage were significantly correlated (P = 0.002); however, miR-141 expression difference between the tumor and polyp samples was not significant (p = 0.6).<br>
<br>
<strong><em>Conclusions:</em></strong> The DNA methylation status of the miR-200c/141 cluster could serve as a progression marker from benign polyps to colorectal cancer.</div>
Colonic Polyps, Colorectal Cancer, DNA methylation, MiR-141
208
215
http://rbmb.net/browse.php?a_code=A-10-313-1&slc_lang=en&sid=1
Zahra
Taheri
zahra_taheri_2005@yahoo.com
10031947532846006676
10031947532846006676
No
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Hamid
Asadzadeh Aghdaei
hamid.assadzadeh@gmail.com
10031947532846006677
10031947532846006677
No
Basic and Molecular Epidemiology of Gastroenterology Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Shiva
Irani
shi_irani@yahoo.com
10031947532846006678
10031947532846006678
Yes
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Mohammad Hossein
Modarressi
modaresi@tums.ac.ir
10031947532846006679
10031947532846006679
No
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Zahra
Noormohammadi
marjannm@yahoo.com
10031947532846006680
10031947532846006680
No
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.