Reports of Biochemistry and Molecular Biology
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Basic Sciences
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1
admin
2322-3480
2322-3480
10.61186/rbmb
en
jalali
1398
4
1
gregorian
2019
7
1
8
2
online
1
fulltext
en
Pattern of ABCC Transporter Gene Expression in Pediatric Patients with Relapsed Acute Lymphoblastic Leukemia
زیست شناسی ملکولی
Molecular Biology
مقالات اصلی
Original Article
<div style="text-align: justify;"><strong><em>Background:</em></strong> Abnormal expression of <em>ABCC</em> transporter genes has been associated with treatment failure in pediatric patients with acute lymphoblastic leukemia (ALL). The aim of this study was to evaluate the expression pattern of <em>ABCC1-6</em> and <em>ABCC10</em> genes in Iranian pediatric patients with ALL relapse and determine the potential predictive value of determining ALL relapse from <em>ABCC </em>expression.<br>
<br>
<strong><em>Methods:</em></strong> Patients with ALL were divided into two separate groups, either the case group with relapsed ALL or the control group in which ALL patients have been in progression-free survival for at least 3 years A total of thirty-nine participants (23 with relapsed ALL; 16 controls) were enrolled over 26 months. To determine the levels of <em>ABCC1-6 </em>and <em>ABCC10 </em>transporter gene expression RT-PCR was used. Cumulative doses of the chemotherapy drugs, VCR, DNR and L-ASP, were calculated for each patient.<br>
<br>
<strong><em>Results:</em></strong> Our findings showed elevated expression of <em>ABCC2-6 </em>and decreased expression of <em>ABCC1</em> and <em>ABCC10</em> to be associated with an increased risk of ALL relapse. The mean-fold expression of <em>ABCC2 </em>was significantly increased in the ALL relapse group. Additionally, the expression pattern of the <em>ABCC </em>transporter genes was associated with high doses of three chemotherapy drugs, VCR, DNR and L-ASP.<br>
<br>
<strong><em>Conclusions:</em></strong> Evaluating the expression pattern of <em>ABCC </em>transporter genes may be a potential biomarker for predicting the occurrence of ALL relapse in Iranian pediatric patients and improve cancer prognosis.<br>
</div>
ABC transporters, Childhood, Gene expression, Pattern, Recurrence.
184
193
http://rbmb.net/browse.php?a_code=A-10-315-1&slc_lang=en&sid=1
Narjes
Mehrvar
narjes.mehrvar@gmail.com
10031947532846006650
10031947532846006650
No
Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Hassan
Abolghasemi
pchd1391@gmail.com
10031947532846006651
10031947532846006651
No
Pediatric Congenital Hematologic Disorders Research Center, Research Institute for Children's Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mohammad Reza
Rezvany
mohrezrez@yahoo.com
10031947532846006652
10031947532846006652
No
Hematology department, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran. & Department of Oncology-Pathology, Immune and Gene Therapy Lab, Cancer Center Karolinska (CCK), Karolinska University Hospital Solna and Karolinska Institutet, Stockholm, Sweden.
Mohammad Esmaeil
Akbari
crccbils@gmail.com
10031947532846006653
10031947532846006653
No
Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Javad
Saberynejad
javad.saberynejad@gmail.com
10031947532846006654
10031947532846006654
No
AJA University of Medical Sciences, Tehran, Iran.
Azim
Mehrvar
drazimmehrvar@yahoo.com
10031947532846006655
10031947532846006655
No
AJA University of Medical Sciences, Tehran, Iran. & Mahak Hematology Oncology Research Center (Mahak-HORC), Mahak Hospital, Tehran, Iran.
Mohammad Ali
Ehsani
maehsani@gmail.com
10031947532846006656
10031947532846006656
No
Bahrami Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Mahyar
Nourian
mahyarnourian1369@gmail.com
10031947532846006657
10031947532846006657
No
Mahak Hematology Oncology Research Center (Mahak-HORC), Mahak Hospital, Tehran, Iran.
Ibrahim
Qaddoumi
ibrahim.qaddoumi@stjude.org
10031947532846006658
10031947532846006658
No
Department of Global Pediatric Medicine, St. Jude Children’s Research Hospital, Memphis, TN USA.
Abolfazl
Movafagh
movafagh_a@yahoo.com
10031947532846006659
10031947532846006659
Yes
Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. & Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran