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'); Reports of Biochemistry and Molecular Biology rbmb.net Basic Sciences http://rbmb.net 1 admin 2322-3480 2322-3480 10.61882/rbmb en jalali 1398 7 1 gregorian 2019 10 1 8 3 online 1 fulltext
en Production of Dextran from Locally Lactobacillus Spp. Isolates میکروب شناسی Microbiology مقالات اصلی Original Article <div style="text-align: justify;"><em><strong>Background:</strong></em>Dextran is a commercially available bacterial exopolysaccharide (EPS) with several industrial applications in the food industry and in the biomedical industry as an adjuvant, emulsifier, carrier, and stabilizer. The production of dextran at the industrial level occurs through the fermentation of a sucrose-rich medium. Research to optimize dextran production has found that the yield of dextran varies depending the specific conditions for production. The aim of this study was to produce dextran and establish the optimal conditions for dextran biosynthesis from different Lactobacillus species isolated from healthy vaginal and infant stool samples.<br> <em><strong>Methods:</strong></em>Lactobacillus spp. were isolated and identified from vaginal and infant stool samples via the VITEK 2 system. The presence of dextran biosynthesis from the different Lactobacillus spp. isolates was determined by a screening test for mucoid colonies and confirmed via theethanol precipitation method. To optimize for the maximum yield of dextran, the effects of various parameters such as temperature, incubation time, pH, inoculum size, aeration, and sucrose concentration were examined.<br> <strong><em>Results:</em></strong>All Lactobacillus spp. isolates were able to produce dextran. The optimal conditions for dextran production was at 24 hours of incubation at 30 &deg;Cwith 15% sucrose, 4% inoculation size at pH 7.0 in aerobic conditions. This yielded a dextran dry weight of 580 mg/100 mL.<br> <strong><em>Conclusions: </em></strong>Dextran production from Lactobacillus species isolates vagina and infant stool had the ability to produce dextran. Conclusions:The DNA methylation status of the miR-200c/141 cluster could serve as a progressionmarker frombenignpolypstocolorectalcancer.</div> Dextran, Lactobacillusspp, Optimum Conditions, Precipitation 278 286 http://rbmb.net/browse.php?a_code=A-10-319-2&slc_lang=en&sid=1 Ali Jumma Kareem alialsahb555@gmail.com 100319475328460017362 100319475328460017362 No Department of Biology-College of Science, Mustansiriyah University, Baghdad, Iraq. Jehan Abdul Sattar Salman Dr.jehan@uomustansiriyah.edu.iq 100319475328460017363 100319475328460017363 Yes Department of Biology-College of Science, Mustansiriyah University, Baghdad, Iraq.