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en Determining the Effect of Amino Acids on the Allergenic Activity of Pollen Extracts ایمنی شناسی Immunology مقالات اصلی Original Article <div style="text-align: justify;">Background: The diagnosis and treatment of allergic diseases require high quality pollen allergen extracts for reliable test results and effective treatments. The quality of the pollen allergen extracts is influenced by pharmacologically inert ingredients, such as stabilizers which are added to prevent the degradation of the allergenic activity. This study was conducted to develop a stabilizer formulation in order to protect the allergenic activity of the pollen’s extracts. Methods: Pine and orchard grass pollen allergen extracts were incubated for 40 days at 37 °C. The effects of chemicals were examined via inhibition ELISA on days 7, 14, 21, 28, and 40 to evaluate the ability of the pollen allergen extracts to inhibit specific IgE in the sera of sensitized patients. Results: Our findings showed that the pine pollen and orchard grass allergen extracts treated with Lys/Glu had the best stabilizing effect resulting in a 97% IgE inhibition following the 40 days of incubation. In the non-treatment group, the IgE inhibition decreased to 23% at the end of the 40 days. The orchard grass pollen allergen extracts receiving no treatment decreased to 12% IgE inhibition following the 40-day incubation. Conclusions: Amino acids are able to act as an effective stabilizer for pollen allergen extracts and prevent the degradation of their activity over time. Particularly applying Lys/ Glu in pollen allergenic extracts can protect allergenic activity and potency of the pollen extracts to inhibit specific IgE in human sera.</div> Amino acids, Pollen, Skin Prick Test, Stabilizing. 394 400 http://rbmb.net/browse.php?a_code=A-10-41-3&slc_lang=en&sid=1 Nazila Ariaee Ariaeen931@mums.ac.ir 100319475328460017419 100319475328460017419 No Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Mojtaba Sankian Sankianm@mums.ac.ir 100319475328460017420 100319475328460017420 No Immunology Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran. Abdolreza Varasteh Varastehar@gmail.com 100319475328460017421 100319475328460017421 No Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Mitra Hosseinpour Hoseinpourm1@sbmu.ac.ir 100319475328460017422 100319475328460017422 No Shahid Beheshti University of Medical Sciences, 15 Khordad Hospital, Tehran, Iran. Farahzad Jabbari Jabbarif@mums.ac.ir 100319475328460017423 100319475328460017423 Yes Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.