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en The Gene Expression Profile and DNA Methylation Pattern of CDH1 and DNMT1 Genes in Acute Promyelocytic Leukemia (APL) زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: DNA methylation is an epigenetic modification that has the ability to alter gene expression and function. These epigenetic changes have been associated with the development of cancer. Previous research has found that DNA methylation patterns can predict disease prognosis for patients with Acute Promyelocytic Leukemia (APL). The role of DNMT1 and CDH1 in regulating the extension of cells are studied in this study. Methods: DNA was extracted from peripheral blood samples of APL patients and treated with bisulfite. DNMT1 and CDH1 gene promoter methylation was subsequently analyzed using methylation-specific PCR (MSP). Real-time PCR was used to measure the expression level of DNMT1 and CDH1 genes. Results: Partial methylation of the CDH1 gene promoter was detected in 20% of APL patients and an unmethylated status was detected in 80% of patient samples. Additionally, an unmethylated status in the DNMT1 gene promoter was detected in 100% of APL patient samples. Conclusions: Our study found the CDH1 gene promoter to be unmethylated in almost all APL patients, while the DNMT1 promoter was unmethylated in all APL patients. Furthermore, we observed an increase in both CDH1 and DNMT1 gene expression in APL patients compared to healthy controls. These findings suggest that DNMT1 may not have a specific role in inhibiting CDH1 gene expression in APL. Applying higher resolution techniques would help to better uncover the DNA methylation patterns in patients with APL. Further research is required to determine the role of DNA methylation and CDH1 and DNMT1 gene expression in APL.</div> Acute Promyelocytic Leukemia, CDH1, DNMT, Promoter Methylation. 454 457 http://rbmb.net/browse.php?a_code=A-10-152-2&slc_lang=en&sid=1 Sanaz Zebardast Zebardastsanaz1992@gmail.com 100319475328460017457 100319475328460017457 No Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran. Mehdi Sahmani M.Sahmani@gmail.com 100319475328460017458 100319475328460017458 No Department of Biochemistry, Faculty of Allied Medicine, Qazvin University of Medical Sciences, Qazvin, Iran. Saeed Mohammadi Smohammadi@sina.tums.ac.ir 100319475328460017459 100319475328460017459 No Hematology-Oncology and stem cell transplantation research center, Tehran university of medical sciences, Tehran, Iran. Farshad Foroughi farshad295@yahoo.com 100319475328460017460 100319475328460017460 No Department of Immunology, Faculty of Allied Medicine, Qazvin university of Medical Sciences, Qazvin, Iran. Ali Dehghani Fard Ali_dehghani_fard@yahoo.com 100319475328460017461 100319475328460017461 No Gholhak laboratory of Tehran, Iran. Zahra Mohammadi Heeva-MH.1988@yahoo.com 100319475328460017462 100319475328460017462 No Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran. Sahar Khojastepour Saharkhojaste62@gmail.com 100319475328460017463 100319475328460017463 No Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran. Mehdi Azad m.azad@qums.ac.ir 100319475328460017464 100319475328460017464 Yes Department of Medical laboratory sciences, Faculty of Allied Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.