Reports of Biochemistry and Molecular Biology
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Basic Sciences
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admin
2322-3480
2322-3480
10.61186/rbmb
en
jalali
1399
2
1
gregorian
2020
5
1
9
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online
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The Relationship between KIT Copy Number Variation, Protein Expression, and Angiogenesis in Sporadic Breast Cancer
زیست شناسی ملکولی
Molecular Biology
مقالات اصلی
Original Article
<div style="text-align: justify;"><strong><em>Background:</em></strong> <em>KIT </em>is a protooncogene that encodes for the KIT oncoprotein, which is a transmembrane tyrosine kinase growth factor receptor that holds a critical role in a variety of normal physiological and pathological processes including angiogenesis. <em>KIT </em>has been shown to be involved in tumorigenesis, contributing to the development of gastrointestinal carcinoma and leukemia. A link between KIT overexpression and breast cancer development has previously been reported. In the current study, we explored <em>KIT</em> gene expression and exonic copy number variants (CNV) and the relationship with angiogenesis (CD34) and the clinicopathological features of breast cancer.<br>
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<strong><em>Methods:</em></strong> MLPA technique was used to determine the CNV in 64 breast cancer tumor samples from patients diagnosed with primary sporadic breast cancer. Results were confirmed by quantitative PCR. Expression of KIT and CD34 was determined using immunohistochemistry (IHC).<br>
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<strong><em>Results</em></strong><strong><em>:</em></strong> Our results show that 28.1% of the tumor samples from patients with primary sporadic breast cancer had CNV in the <em>KIT </em>gene. Among the breast tumor samples, 54.7% showed positive <em>KIT</em> expression. The expression of the CD34 angiogenesis marker was reported in 43.8% of the tumor samples as low, 42.2% as moderate and 14.1% as high. A significant correlation between increased CNV of <em>KIT</em> exons, a high level of angiogenesis (CD34) and increased tumor grade was observed (p< 0.05). <br>
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<strong><em>Conclusions:</em></strong> A significant correlation between the <em>KIT</em> CNV and the angiogenesis marker was found. Examining <em>KIT</em> expression and CNV has the potential to function as a biomarker for tyrosine kinase inhibitor drugs in breast cancer.</div>
Angiogenesis, Breast Cancer, CD34, KIT.
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http://rbmb.net/browse.php?a_code=A-10-395-1&slc_lang=en&sid=1
Maryam
Rahimi
maryamrahimi1986@yahoo.com
10031947532846007246
10031947532846007246
No
Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran. & Department of Microbiology, Karaj branch, Islamic Azad University, Karaj, Iran.
Farkhondeh
Behjati
fbehjati@gmail.com
10031947532846007247
10031947532846007247
No
Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
Hamid Reza
Khorram Khorshid
hrkhkh@yahoo.com
10031947532846007248
10031947532846007248
No
Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
Masoud
Karimlou
mkarimlo@yahoo.com
10031947532846007249
10031947532846007249
No
Department of Epidemiology and Biostatistics, Tehran Medical Branch, Islamic Azad University, Tehran, Iran.
Elahe
Keyhani
ekeyhani1058@gmail.com
10031947532846007250
10031947532846007250
Yes
Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran. & Clinical Research Development Center of Rofeideh Rehabilitation Hospital, Tehran, Iran.