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'); Reports of Biochemistry and Molecular Biology rbmb.net Basic Sciences http://rbmb.net 1 admin 2322-3480 2322-3480 10.61882/rbmb en jalali 1393 2 1 gregorian 2014 5 1 2 2 online 1 fulltext
en Identification of Spata-19 New Variant with Expression beyond Meiotic Phase of Mouse Testis Development زیست شناسی سلولی Cell Biology <p style="text-align: justify;"><em><strong>Background:</strong></em> The study of specific genes expressed in the testis is important to understanding testis development and function. Spermatogenesis is an attractive model for the study of gene expression during germ cell differentiation. Spermatogenesis associated-19 (<em>Spata-19</em>) is a recently-identified important spermatogenesis-related gene specifically expressed in testis. Its protein product is involved in sperm cell development and reproduction. In this report we examined the expression of <em>Spata-19</em> mRNA in mouse testis, fetus, and cell lines.</p> <p style="text-align: justify;"><em><strong>Methods:</strong></em> Reverse transcription-polymerase chain reaction (RT-PCR), nested PCR, and PCR-restriction fragment length polymorphism (PCR-RFLP) were used to analyze <em>Spata-19</em> mRNA expression in different stages of mouse testis development, mouse fetus, mouse embryonic fibroblasts (MEF), mouse embryonic stem cells (mESC), Sertoli cells, and NIH/3T3 cells.</p> <p style="text-align: justify;"><em><strong>Results: </strong></em>We identified a novel splice variant of <em>Spata-19</em> in the mouse genome that it is expressed in the fetus and after the meiotic phase of spermatogenesis, and over-expressed in the post-meiotic stage of mouse spermatogenesis. This novel splice variant was absent in five days old mice testis, mESC, MEF, Sertoli, and NIH/3T3 cell lines.</p> <p style="text-align: justify;"><em><strong>Conclusion:</strong></em> The<em> Spata-19</em> has a large novel splice variant in mouse testis that is expressed beyond meiotic phase of testis development. We suggest that this new <em>Spata-19</em> mRNA variant might be involved in mitochondrial maintenance in sperm cells, and might be correlated with androgen secretion &lrm;and male fertility.</p> Fertility, Spata-19‎ new variant, Spermatogenesis, Testis 88 93 http://rbmb.net/browse.php?a_code=A-10-1-27&slc_lang=en&sid=1 Seyedmehdi Nourashrafeddin 10031947532846005995 10031947532846005995 No Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Reza Ebrahimzadeh-Vesal 10031947532846005996 10031947532846005996 No Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Mohammad Hosein Modarressi 10031947532846005997 10031947532846005997 No Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Ali Zekri 10031947532846005998 10031947532846005998 No Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Mohammad Nouri nourim@tbzmed.ac.ir 10031947532846005999 10031947532846005999 Yes Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran - Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.