Reports of Biochemistry and Molecular Biology
rbmb.net
Basic Sciences
http://rbmb.net
1
admin
2322-3480
2322-3480
10.61186/rbmb
en
jalali
1399
7
1
gregorian
2020
10
1
9
3
online
1
fulltext
en
Recognition of (Sesc) for Easy Identification of Staphylococcus Epidermidis and Molecular and Phenotypic Study of Β-Lactam Resistance in Staphylococcus Epidermidis Isolates in Isfahan
میکروب شناسی
Microbiology
مقالات اصلی
Original Article
<div style="text-align: justify;"><strong><em>Background:</em></strong> Not only is it crucial to rapidly detect <em>Staphylococcus epidermidis </em>(<em>S. epidermidis</em>) isolates from a broad range of bacteria, but recognizing resistance agents can greatly improve current diagnostic and therapeutic strategies.<br>
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<strong><em>Methods:</em></strong> The current cross-sectional study investigated 120 clinical isolates from a nosocomial <em>S. epidermidis </em>infection. The isolates were identified using common biochemical tests, and specific <em>S. epidermidis</em> surface protein C (<em>SesC</em>) primers were used to confirm the presence of <em>S. epidermidis</em>. PCR and special primers were used to detect the β-lactamase gene (<em>blaZ</em>). Methicillin resistance was measured using the agar screening method and antibiotic susceptibility was measured by disk diffusion.<br>
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<strong><em>Results:</em></strong> 100 samples were characterized as <em>S. epidermidis </em>using a phenotypic and genotypic methods. From the 100 specimens examined, 80% contained <em>blaZ</em>. According to agar screening, 60% of isolates were methicillin-resistant. <em>S. epidermidis</em> isolates demonstrated the highest resistance to penicillin (93%) and the highest sensitivity to cefazolin <em>(39%)</em><em>.</em><br>
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<strong><em>Conclusions:</em></strong> The increased resistance to β-lactam antibiotics in <em>S. epidermidis</em> isolates is alarming, and certain precautions should be taken by healthcare systems to continuously monitor the antimicrobial pattern of <em>S. epidermidis, </em>so that an appropriate drug treatment can be established.</div>
Antibiotic resistance, β-lactam, Staphylococcus epidermidis
309
314
http://rbmb.net/browse.php?a_code=A-10-460-1&slc_lang=en&sid=1
Parisa
Behshood
10031947532846007579
10031947532846007579
No
Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Elahe
Tajbakhsh
elahe.tajbakhsh64@yahoo.com
10031947532846007580
10031947532846007580
Yes
Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Hassan
Momtaz
10031947532846007581
10031947532846007581
No
Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran