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en Protective Effects of Quercetin and Melatonin on Indomethacin Induced Gastric Ulcers in Rats بیوشیمی Biochemistry مقالات اصلی Original Article <div style="text-align: justify;">Background: Medications to prevent the development of NSAID-induced gastric ulcers have a large range of unpleasant side effects. Recent efforts have been focused on determining safer alternative non-toxic and natural forms of anti-ulcer treatments. Methods: Twenty-four male rats were divided into 4 groups: 1: control group that received no treatment; 2: the indomethacin-treated group that received 20 mg/kg of indomethacin for 2 days to induce the development of gastric ulcers; 3: quercetin-treated group that in addition to the indomethacin treatment, received 50 mg/kg of quercetin 6 hours after and then daily for 14 days and; 4: the melatonin-treated group which received 20 mg/kg of melatonin 6 hours after each indomethacin treatment and then daily for 14 days. All drugs were administered orally. The following parameters were assessed in each group: mean ulcer index of gastric tissue, gastric acid volume and pH, oxidative stress markers: malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH), inflammatory markers: PGE-2, TNF-α, and IL-10, nitric oxide (NO) levels and the relative gene expression of BAX, BCL-2 and COX-2 by real time PCR. Results: Our findings revealed that the indomethacin-treated group had a significantly increased (p< 0.05) ulcer index, gastric acid volume, and elevated levels of stress, inflammatory, and apoptotic markers compared to controls. In the groups that received quercetin or melatonin, these factors were all significantly decreased (p< 0.05). Between quercetin and melatonin, there was no significant difference in their gastroprotective effect. Conclusions: Both quercetin and melatonin had protective antioxidant, anti-inflammatory and antiapoptotic activity against indomethacin-induced gastric ulcers.</div> Gastric ulcer, Indomethacin, Melatonin, Quercetin 278 290 http://rbmb.net/browse.php?a_code=A-10-310-2&slc_lang=en&sid=1 Marwa Sayed Abdel-Tawab drmarwasayed2016@gmail.com 100319475328460017658 100319475328460017658 Yes Medical Biochemistry Department, Faculty of Medicine, Beni-Suef University, Egypt Ola Mostafa Tork 100319475328460017659 100319475328460017659 No Basic Medical Science Department, Faculty of Medicine, Princess Nourah bint Abdulrahman University, Kingdom of Saudi Arabia & Department of Medical Physiology, Faculty Gomaa Mostafa-Hedeab 100319475328460017660 100319475328460017660 No Pharmacology Department, Medical college, Jouf University, Kingdom of Saudi Arabia & Pharmacology Department- Faculty of Medicine, Beni-Suef University, Egypt. Manal Ewaiss Hassan 100319475328460017661 100319475328460017661 No Medical Biochemistry Department, Faculty of Medicine, Beni-Suef University, Egypt & Medical Biochemistry Department, Faculty of Medicine, Al Jouf University, Kingdom of Saudi Arabia Dalia Azmy Elberry 100319475328460017662 100319475328460017662 No Department of Medical Physiology, Faculty of Medicine, Cairo University, Egypt.