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en Comparison of Osteogenic Potential of Phenytoin with Dexamethasone in Cultured Dental Pulp Stem Cells زیست شناسی سلولی Cell Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: One of the adverse effects of phenytoin (diphenylhydantoin, DPH) is enlargement of facial features. Although there are some reports on anabolic action of phenytoin on bone cells, the osteogenic potential of DPH on mesenchymal stem cells has not been studied. The purpose of this study was to evaluate the osteogenic potential of DPH on dental pulp stem cells (DPSCs). Methods: Human DPSCs were isolated and characterized by flow cytometry; presence of CD29 and CD44 and absence of CD34 and CD45 were performed to confirm the mesenchymal stem cells. Isolated DPSCs were differentiated either in conventional osteogenic medium with Dexamethasone or medium containing different concentration of phenytoin (12.5, 25, 100, and 200 µM). The osteogenic differentiation evaluated by performing western blot test for Runt-related transcription factor 2 (RUNX2), osteopontin and alkaline phosphatase (ALP) also alizarin red S staining to measure the mineralization of cells. Results: Our results showed morphological changes and mineralization of DPSCs by using DPH were comparable with dexamethasone. Moreover, western blot results of DPH group showed significant increase of ALP, <a name="OLE_LINK1">RUNX2</a> and osteopontin (OSP) in comparison with control. Conclusions: The data of present study showed the osteogenic activity of phenytoin, considering as an alternative of dexamethasone for inducing osteogenic differentiation of dental pulp stem cells.</div> Dental Pulp Stem Cells, Osteogenic Differentiation, Phenytoin. 331 337 http://rbmb.net/browse.php?a_code=A-10-538-1&slc_lang=en&sid=1 Mitra Asgharian-Rezaee 100319475328460017683 100319475328460017683 No Department of Toxicology and Pharmacology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran & Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran. Raheleh Alipour-Farmad 100319475328460017684 100319475328460017684 No Department of Toxicology and Pharmacology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran Zahra Tayarani-Najaran tayaraninz@mums.ac.ir 100319475328460017685 100319475328460017685 Yes Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.