Reports of Biochemistry and Molecular Biology
rbmb.net
Basic Sciences
http://rbmb.net
1
admin
2322-3480
2322-3480
10.61186/rbmb
en
jalali
1393
7
1
gregorian
2014
10
1
3
1
online
1
fulltext
en
TLR4 and TLR2 Expression in Biopsy Specimens from Antral and Corporal Stomach Zones in Helicobacter pylori Infections
میکروب شناسی
Microbiology
<p style="text-align: justify;"><em><strong>Background:</strong></em> It is not yet known which types of Toll-like receptors (TLRs) are most effective in Helicobacter pylori (H. pylori) recognition. It is also not known which gastric zones have the most prominent roles in TLR-mediated bacterial recognition. The aim of this work was to analyze the expression of TLR2 and TLR4 in biopsy specimens from H. pylori-infected patients.</p>
<p style="text-align: justify;"><strong><em>Methods:</em></strong> Thirty-eight patients with gastrointestinal disorders were divided into four groups in this study. The groups were: (A) H. pylori infection and peptic ulcer (n=15), (B) peptic ulcer only (n=5), (C) H. pylori infection only (n=10) and (D) control, with neither H. pylori infection nor peptic ulcer (n=8). Biopsy specimens from sites of redness or atrophic mucosa from gastric antrum and body in patients with gastritis were collected. RNAs from the antrum and body specimens were isolated. TLR2 and TLR4 mRNA expression was assessed by RT-PCR and quantified as densitometric ratios of TLR2 and TLR4/β-actin mRNA.</p>
<p style="text-align: justify;"><strong><em>Results: </em></strong>In the antral zones of H. pylori-infected patients (Groups A and C) TLR2 and TLR4 expression was significantly greater than in uninfected patients (Groups B and D) regardless of peptic ulcers (p < 0.05). In the gastric body samples TLR2 expression was significantly greater in Group C (H. pylori infection only) than in Group B (peptic ulcer only) and TLR4 expression was significantly greater in group A (H. pylori infection and peptic ulcer) than in Group B (peptic ulcer only) (p < 0.05). No significant differences in expression of TLR4 and TLR2 were observed between samples from the antrum and body in same groups.</p>
<p style="text-align: justify;"><em><strong>Conclusions:</strong></em> We conclude that H. pylori infection leads to significant increase in TLR2 and TLR4 molecules expression in antral region related to the control group. Considering the stimulatory effect of H. pylori on TLRs expression in the gastric tissue, we assume that colonization of H. pylori infection might occurs more in the gastric antral region than in the gastric body.</p>
Helicobacter pylori, Peptic ulcer, TLR2, TLR4, Toll-like receptors
29
37
http://rbmb.net/browse.php?a_code=A-10-1-34&slc_lang=en&sid=1
Mohammad Reza
Khakzad
khakzadmr911@mums.ac.ir
10031947532846006028
10031947532846006028
Yes
Department of Immunology, Zakariya Research Center, Mashhad Branch, Islamic Azad University, Mashhad, Iran - Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Ahmad
Saffari
10031947532846006029
10031947532846006029
No
Internal Medicine Department, Mashhad Branch, Aria Hospital, Islamic Azad University, Mashhad, Iran
Niloofar
Mohamadpour
10031947532846006030
10031947532846006030
No
Department of Microbiology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Mojtaba
Sankian
10031947532846006031
10031947532846006031
No
Immunobiochemistry lab, Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Abdolreza
Varasteh
10031947532846006032
10031947532846006032
No
Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Farhad
Salari
10031947532846006033
10031947532846006033
No
Immunobiochemistry lab, Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Mojtaba
Meshkat
10031947532846006034
10031947532846006034
No
Department of Immunology, Zakariya Research Center, Mashhad Branch, Islamic Azad University, Mashhad, Iran