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'); Reports of Biochemistry and Molecular Biology rbmb.net Basic Sciences http://rbmb.net 1 admin 2322-3480 2322-3480 10.61882/rbmb en jalali 1393 7 1 gregorian 2014 10 1 3 1 online 1 fulltext
en Hemolytic and Cytotoxic Properties of Saponin Purified from Holothuria leucospilota Sea Cucumber زیست شناسی سلولی Cell Biology <p style="text-align: justify;"><em><strong>Background:</strong></em> Holothuroids (sea cucumbers) are members of the phylum echinodermata, which produce saponins. Saponins exhibit a wide spectrum of pharmacological and biological activities. In this study, we isolated the crude saponins from the body wall of the dominant Iranian species of sea cucumber, Holothuria leucospilota (H. leucospilota). The purpose of this study was to confirm the presence of saponins in the Persian Gulf H. leucospilota and study the hemolytic and cytotoxic activities of these compounds.</p> <p style="text-align: justify;"><em><strong>Methods:</strong></em> The body wall of sea cucumber was dried and powdered and the crude saponins were isolated using various solvents. The crude saponins were further purified by column chromatography using HP-20 resin. The foam test, Thin Layer Chromatography (TLC), hemolytic assay, and Fourier Transform Infrared Spectroscopy (FTIR) confirmed the presence of saponins. Cytotoxicity was analyzed using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay on A549 cells, a human lung cancer cell line.</p> <p style="text-align: justify;"><em><strong>Results:</strong></em> The foam test, hemolytic assay, and TLC supported the presence of saponin compounds in the 80% ethanol fraction of H. leucospilota. The infrared (IR) spectrum of the extract showed hydroxyl (-OH), alkyl (C-H), ether (C-O) and ester (&ndash;C=O) absorption characteristic of teriterpenoid saponins. The C-O-C absorption indicated glycoside linkages to the sapogenins. The crude saponin extracted from sea cucumber was cytotoxic to A549 cells.</p> <p style="text-align: justify;"><em><strong>Conclusion:</strong></em> The 80% ethanol fraction of saponin isolated from H. leucospilota exhibited hemolytic activity and offers promise as an anti-cancer candidate.</p> Cytotoxicity assay, Hemolytic assay, Holothuria leucospilota, Saponin, Sea cucumber 43 50 http://rbmb.net/browse.php?a_code=A-10-1-36&slc_lang=en&sid=1 Mozhgan Soltani soltani_m4@yahoo.com 10031947532846006041 10031947532846006041 Yes Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Kazem Parivar 10031947532846006042 10031947532846006042 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Javad Baharara 10031947532846006043 10031947532846006043 No Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran Mohammad Amin Kerachian 10031947532846006044 10031947532846006044 No Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Javad Asili 10031947532846006045 10031947532846006045 No Department of Pharmacognosy, School of Pharmacy, Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran