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en RNA Sequencing of Early-Stage Gastric Adenocarcinoma Reveals Multiple Activated Pathways and Novel Long Non-Coding RNAs in Patient Tissue Samples زیست شناسی سلولی Cell Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: Gastric cancer is among the most common cancers worldwide that currently lacks effective diagnostic biomarkers and therapeutic targets. Next-generation RNA sequencing is a powerful tool that allows rapid and accurate transcriptome-wide profiling to detect differentially expressed transcripts involved in normal biological and pathological processes. Given the function of this technique, it has the potential to identify new molecular targets for the early diagnosis of disease, particularly in gastric adenocarcinoma. Methods: In this study, whole-transcriptome analysis was performed with RNA sequencing on tumoral and non-tumoral tissue samples from patients with early-stage gastric cancer. Gene ontology and pathway enrichment analysis were used to determine the main function of the specific genes and pathways present in tissue samples. Results: Analysis of the differentially expressed genes revealed 5 upregulated and 234 downregulated genes in gastric cancer tissues. Pathway enrichment analysis revealed significantly dysregulated signalling pathways, including those involved in gastric acid secretion, drug metabolism and transporters, molecular toxicology, O-linked glycosylation of mucins, immunotoxicity, metabolism of xenobiotics by cytochrome P450, and glycosylation. We also found novel downregulated non-coding RNAs present in gastric cancer tissues, including GATA6 antisense RNA 1, antisense to LYZ, antisense P4HB, overlapping ACER2, long intergenic non-protein coding RNA 2688 (LINC02688) and uncharacterized LOC25845 (PP7080). Conclusions: The transcriptomic data found in this study illustrates the power of RNA-sequencing in discovering novel genes &lrm;and tumorigenic pathways involved in human carcinogenesis. The anomalies present in these genes may serve as promising tools for the development of accurate diagnostic biomarkers for the detection of early-stage gastric cancer.</div> Gastric Cancer, LncRNAs Signalling Pathway, RNA-sequencing. 478 489 http://rbmb.net/browse.php?a_code=A-10-587-1&slc_lang=en&sid=1 Sadegh Fattahi 100319475328460017774 100319475328460017774 No Student Research Committee, Babol University of Medical Sciences, Babol, Iran.‎ & Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran. & North Research Center of Pasteur Institute, Amol, Iran. Novin Nikbakhsh 100319475328460017775 100319475328460017775 No Department of Surgery, Rouhani hospital Babol University of Medical Sciences, Babol, Iran. Hassan Taheri ‎ 100319475328460017776 100319475328460017776 No Department of Internal Medicine, Rouhani hospital Babol University of Medical Sciences, Babol, Iran. Mohammad Ranaee 100319475328460017777 100319475328460017777 No Department of Pathology, Rouhani hospital, Babol University of Medical Sciences, Babol, Iran. Haleh Akhavan-Niaki 100319475328460017778 100319475328460017778 Yes Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran. & Department of Genetics, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.