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en Prevalence of Virulence Genes and Antibiotic Resistance Pattern in Enterococcus Faecalis Isolated from Urinary Tract Infection in Shahrekord, Iran میکروب شناسی Microbiology مقالات اصلی Original Article Background: This study aims to specify the antimicrobial resistance pattern and virulence genes of Enterococcus faecalis isolated from urinary tract infections in Shahrekord, Iran. Methods: Urine samples of 1000 people suspected of having urinary tract infections referred to Shahrekord medical diagnostic laboratories were examined. Biofilm assays were performed by microtiter plate test through reading the OD490. Polymerase Chain Reaction (PCR) was applied to study the virulence factors. Results: Enterococcus faecalis was detected in 60 samples. After performing microbiological tests, all samples were positive in the molecular analysis. Strong, moderate and weak biofilm reactions reported 66.67%, 25%, and 8.33% respectively. The most resistance reported to cotrimoxazole, vancomycin and amikacin and the lowest resistance to nitrofurantoin (8.33%) was reported. Statistical analysis with Fisher's exact test showed a statistically significant relationship between biofilm production and resistance to cotrimoxazole, vancomycin and cefotaxime. Prevalence of efe A, ace, gel E, esp, cyl M, agg, cyl A and cyl B in strong biofilm formation isolates was reported 100%, 87.5%, 82%, 62.5%, 55%, 37.5% 25% and 22.5% respectively. There was a significant relationship between the frequency of efa A and strong biofilm reaction. Conclusions: The presence of E. faecalis strains resistant to co-trimoxazole and vancomycin and present of some virulence factors is alarming the researchers. Since antibiotic resistance genes are probably transmitted among enterococci, and Staphylococci, controlling infections made by enterococci as well as the appropriate administration of antibiotics could treat the nosocomial infections effectively. Antibiotic Resistance, Enterococcus faecalis, Urinary Tract Infection, Virulence genes. 50 59 http://rbmb.net/browse.php?a_code=A-10-620-1&slc_lang=en&sid=1 Roya Jafarzadeh Samani 100319475328460012724 100319475328460012724 No Department of Microbiology, Faculty of Basic Science, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran. Elahe Tajbakhsh ee_tajbakhsh@yahoo.com 100319475328460012725 100319475328460012725 Yes Department of Microbiology, Faculty of Basic Science, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran. Hassan Momtaz 100319475328460012726 100319475328460012726 No Department of Microbiology, Faculty of Basic Science, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran. Mohsen Kabiri Samani 100319475328460012727 100319475328460012727 No Internal Medicine Department, Shahrekord University of Medical Siences. Shahrekord, Iran & Internal Medicine Department, Shahrekord University of Medical Sciences. Shahrekord, Iran.