die('
Site is under construction
Dear site users
Site is under construction.
The site will be ready in less than 24 hours.
We are sorry for the inconvenience.
www.yektaweb.com
');
Reports of Biochemistry and Molecular Biology
rbmb.net
Basic Sciences
http://rbmb.net
1
admin
2322-3480
2322-3480
10.61882/rbmb
en
jalali
1394
2
1
gregorian
2015
5
1
3
2
online
1
fulltext
en
The Anti-Proliferative and Anti-Angiogenic Effect of the Methanol Extract from Brittle Star
زیست شناسی سلولی
Cell Biology
<p style="text-align: justify;"><em><strong>Background: </strong></em>Anti-angiogenic therapy is a crucial step in cancer treatment. The discovery of new anti-angiogenic compounds from marine organisms has become an attractive concept in anti-cancer therapy. Because little data correlated to the pro- and anti-angiogenic efficacies of <em>Ophiuroidea</em>, which include brittle star, the current study was designed to explore the anti-angiogenic potential of brittle star methanol extract<em> in vitro</em> and <em>in vivo</em>.</p>
<p style="text-align: justify;"><em><strong>Methods:</strong></em> The anti-proliferative effect of brittle star extract on A2780cp cells was examined by MTT assays, and transcriptional expression of VEGF and b-FGF was evaluated by RT-PCR. In an in vivo model, 40 fertilized Ross eggs were divided into control and three experimental groups. The experimental groups were incubated with brittle star extract at concentrations of 25, 50 and 100 µg/ml, and photographed by photo-stereomicroscopy. Ultimately, numbers and lengths of vessels were measured by Image J software. Data were analyzed with SPSS software (p<0.05).</p>
<p style="text-align: justify;"><em><strong>Results:</strong></em> Results illustrated that the brittle star extract exerted a dose- and time-dependent anti-proliferative effect on A2780cp cancer cells. In addition, VEGF and b-FGF expression decreased with brittle star methanol extract treatment. Macroscopic evaluations revealed significant changes in the second and third experimental group compared to controls (p<0.05).</p>
<p style="text-align: justify;"><em><strong>Conclusion:</strong></em> These finding revealed the anti-angiogenic effects of brittle star methanol extract <em>in vitro</em> and <em>in vivo</em> confer novel insight into the application of natural marine products in angiogenesis-related pathologies.</p>
Angiogenesis, Anti-proliferative, Brittle star, Marine organisms, Ovarian cancer
68
75
http://rbmb.net/browse.php?a_code=A-10-1-40&slc_lang=en&sid=1
Javad
Baharara
baharara@yahoo.com
10031947532846006061
10031947532846006061
Yes
Research Center for Animal Development Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Elaheh
Amini
10031947532846006062
10031947532846006062
No
Faculty of Biological sciences, Developmental Biology, Kharazmi University, Tehran, Iran
Marzieh
Mousavi
10031947532846006063
10031947532846006063
No
Cell & Developmental Biology Research center for Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran