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en Dysregulation of Key Proteinases in Aspergillus fumigatus Induced by Blood Platelets میکروب شناسی Microbiology مقالات اصلی Original Article Background: Aspergillus fumigatus is the most common species causing invasive aspergillosis (IA), a life-threatening infection with more than 80% mortality. Interactions between A. fumigatus and human blood platelets lead to intravascular thrombosis and localized infarcts. To better understand A. fumigatus pathogenesis, we aimed to analyze the genetic basis of interactions between the pathogen and blood platelets. Methods: A bioinformatic pipeline on microarray gene expression dataset, including analysis of differentially expressed genes (DEGs) using Limma R package and their molecular function, as well as biological pathways identification, was conducted to find the effective genes involved in IA. In the wet phase, the gene expression patterns following fungal exposure to blood platelets at 15, 30, 60, and 180 min were evaluated by quantitative reverse transcriptase-PCR analysis. Results: Three genes encoding aspartic endopeptidases including (Pep1), (Asp f 13), and (β-glucanase) were the standing candidates. The invasion-promoting fungal proteinase-encoding genes were down-regulated after 30 min of hyphal incubation with blood platelets, and then up-regulated at 60 and 180 min, although only Pep1 was greater than the control at the 60and 180 min time points. Also, the same genes were downregulated in more the clinical isolates relative to the standard strain CBS 144.89. Conclusions: Our findings delineate the possible induction of fungal-encoded proteinases by blood platelets. This provides a new research line into A. fumigatus’ molecular pathogenesis. Such insight into IA pathogenesis might also guide researchers toward novel platelet-based therapies that involve molecular interventions, especially in IA patients. Aspergillus fumigatus, Blood Platelets, Gene Expression, Microarray Analysis, Proteinases. 95 104 http://rbmb.net/browse.php?a_code=A-10-641-1&slc_lang=en&sid=1 Bahareh Arghavan 100319475328460012756 100319475328460012756 No Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Mohammad Shafiee 100319475328460012757 100319475328460012757 No Department of Medical Laboratory Sciences, Khomein University of Medical Sciences, Khomein, Iran & Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran. Seyed Jamal Hashemi 100319475328460012758 100319475328460012758 No Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Sadegh Khodavaisy 100319475328460012759 100319475328460012759 No Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Nazanin Hosseinkhan 100319475328460012760 100319475328460012760 No Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran. Mojtaba Didehdar 100319475328460012761 100319475328460012761 No Department of Parasitology and Mycology, School of Medicine, Arak University of Medical Sciences, Arak, Iran. Muhammad Getso 100319475328460012762 100319475328460012762 No Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran & Department of Medical Microbiology and Parasitology, College of Health Sciences, Bayero University, Kano, Nigeria. Ayatollahi Aliasghar 100319475328460012763 100319475328460012763 No Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran. Sassan Rezaie srezaie@tums.ac.ir 100319475328460012764 100319475328460012764 Yes Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.