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Reports of Biochemistry and Molecular Biology
rbmb.net
Basic Sciences
http://rbmb.net
1
admin
2322-3480
2322-3480
10.61882/rbmb
en
jalali
1394
2
1
gregorian
2015
5
1
3
2
online
1
fulltext
en
Expression of TGF-β3 in Isolated Fibroblasts from Foreskin
زیست شناسی سلولی
Cell Biology
<p style="text-align: justify;"><em><strong>Background:</strong></em> The multifunctional transforming growth factor beta (TGF-β) is a glycoprotein that exists in three isoforms. TGF-β3 expression increases in fetal wound healing and reduces fibronectin and collagen I and III deposition, and also improves the architecture of the neodermis which is a combination of blood vessels and connective tissue during wound healing. Fibroblasts are key cells in the wound healing process. TGF-β3 plays a critical role in scar-free wound healing and fibroblast actions in the wound healing process. The aim of this study was to express the TGF-β3 gene (tgf-b3) in human foreskin fibroblasts (HFF’s).</p>
<p style="text-align: justify;"><em><strong>Methods: </strong></em>We obtained HFF’s from a newborn and a primary fibroblast culture was prepared. The cells were transfected with TGF-β3-pCMV6-XL5 plasmid DNA by both lipofection and electroporation. Expression of TGF-β3 was measured by enzyme-linked immunosorbent assay (ELISA).</p>
<p style="text-align: justify;"><em><strong>Results: </strong></em>The highest TGF-β3 expression (8.3-fold greater than control) was obtained by lipofection after 72 hours using 3 µl of transfection reagent. Expression was 1.4-fold greater than control by electroporation.</p>
<p style="text-align: justify;"><em><strong>Conclusions:</strong></em> In this study, we successfully increased TGF-β3 expression in primary fibroblast cells. In the future, grafting these transfected fibroblasts onto wounds can help the healing process without scarring.</p>
Fibroblasts, Gene expression, TGF-β3
76
81
http://rbmb.net/browse.php?a_code=A-10-1-41&slc_lang=en&sid=1
Mahnaz
Mahmoudi Rad
10031947532846006064
10031947532846006064
No
Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Niki
Mahmoudi Rad
10031947532846006065
10031947532846006065
No
Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Yasaman
Mirdamadi
yasaman.mirdamadi@med.ovgu.de
10031947532846006066
10031947532846006066
Yes
Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran - Department of Dermatology and Venereology, Otto-von Guericke University Magdeburg, Magdeburg, Germany