die(' Site is under construction

Dear site users

Site is under construction.

The site will be ready in less than 24 hours.

We are sorry for the inconvenience.

www.yektaweb.com

'); Reports of Biochemistry and Molecular Biology rbmb.net Basic Sciences http://rbmb.net 1 admin 2322-3480 2322-3480 10.61882/rbmb en jalali 1400 1 1 gregorian 2021 4 1 10 1 online 1 fulltext
en Identification of Trichomonas Vaginalis Genotypes Using by Actin Gene and Molecular Based Methods in Southwest of Iran میکروب شناسی Microbiology مقالات اصلی Original Article <div style="text-align: justify;"><strong><em>Background:</em></strong> <a name="_Hlk68880662"><em>Trichomonas vaginalis </em></a>(<em>T. vaginalis</em>) is a sexually transmitted protozoan parasite and the causative agent of trichomoniasis. The genetic characterization of <em>T. vaginalis</em> isolates shows notable genetic variation in this parasite. In the present study, we aimed to identify the <em>T. vaginalis</em> genotypes based on analyzing of actin gene in women specimens referred to health centers of Ilam city, southwest Iran<span dir="RTL">.</span><br> <strong><em>Methods:</em></strong> A total of 1765 female samples were collected from gynecology clinics in the city of Ilam. DNA was extracted from positive samples and nested polymerase chain reaction (Nested PCR) was used to amplify the actin gene. Then, partial sequencing and genotyping of the actin gene was performed. A phylogenetic tree was drawn using the detected genotypes of <em>T. vaginalis</em> and reference sequences.<br> <strong><em>Results:</em></strong> Twenty-one of the 1765 urine and vaginal samples were positive for <em>T. vaginalis</em>. All infected individuals were married and their age in years was between 25 to 34. Further, the majority of infected women had cervical lesions, patchy erythema, and white color discharge. According to sequencing analysis, the isolates were identified as genotype G (n= 8) and genotype E (n= 2).<br> <strong><em>Conclusions:</em></strong> From the collected samples, we were able to distinguish at least two genotypes (G and E) of <em>T. vaginalis</em>. However, lesser is known about these genotypes in the city of Ilam. Further studies with a higher number of isolates should be performed in order to understand the implications of these results in this region.</div> Actin gene, Genotypes, Ilam, Iran, Trichomonas vaginalis. 135 143 http://rbmb.net/browse.php?a_code=A-10-656-1&slc_lang=en&sid=1 Maryam Alikhani alikhanimaryam777@gmail.com 100319475328460012785 100319475328460012785 No Department of Medical Parasitology, Faculty of Allied Medical Sciences, Ilam University of Medical Sciences, Ilam, Iran & Zoonotic Diseases Research Center, Ilam University of Medical Sciences, Ilam, Iran. Reza Saberi reza.sab.68@gmail.com 100319475328460012786 100319475328460012786 No Mazandaran University of Medical Sciences, Sari, Iran Seyed Abdollah Hosseini 100319475328460012787 100319475328460012787 No Toxoplasmosis Research Center, Department of Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. Fatemeh Rezaei 100319475328460012788 100319475328460012788 No Islamic Azad University, Chalus Branch, Chalus, Iran. Abdol satar Pagheh 100319475328460012789 100319475328460012789 No Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran. Asad Mirzaei mirzaei-as@medilam.ac.ir 100319475328460012790 100319475328460012790 Yes Department of Medical Parasitology, Faculty of Allied Medical Sciences, Ilam University of Medical Sciences, Ilam, Iran & Zoonotic Diseases Research Center, Ilam University of Medical Sciences, Ilam, Iran.