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en Evaluation of Oxidative Stress, Apoptosis, and Expression of MicroRNA-208a and MicroRNA-1 in Cardiovascular Patients زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: MicroRNA expression signature and reactive oxygen species (ROS) production have been associated with the development of cardiovascular diseases (CVDs). This study aimed to evaluate oxidative stress, inflammation, apoptosis, and the expression of miRNA-208a and miRNA-1 in cardiovascular patients. Methods: The study population included four types of patients (acute coronary syndromes (ACS), myocardial infarction (MI), arrhythmia, and heart failure (HF)), with 10 people in each group, as well as a control group. Quantitative real-time PCR was performed to measure mir-208 and miR-1 expression, the mRNAs of inflammatory mediators (TNFα, iNOS/eNOS), and apoptotic factors (Bax and Bcl2). XOX, MDA, and antioxidant enzymes (CAT, SOD, and GPx) were measured by ZellBio GmbH kits by an ELISA Reader. Results: The results showed significant decreases in the activity of antioxidant enzymes (CAT, SOD, and Gpx) and a significant increase in the activity of the MDA and XOX in cardiovascular patients. Significant increases in IL-10, iNos, iNOS / eNOS, and TNF-α in cardiovascular patients were also observed. Also, a significant increase in the expression of miR-208 (HF> arrhythmia> ACS> MI) and a significant decrease in the expression of miR-1 (ACS> arrhythmia> HF> MI) were found in all four groups in cardiovascular patients. Conclusions: The results showed increases in oxidative stress, inflammation, apoptotic factors, and in the expression of miR-208a in a variety of cardiovascular patients (ACS, MI, arrhythmia, and HF). It is suggested that future studies determine the relationships that miR-1, miR-208, and oxidative stress indices have with inflammation and apoptosis.</div> Apoptosis, Cardiovascular diseases, Inflammation, microRNA-208a, microRNA-1, Oxidative stress. 183 196 http://rbmb.net/browse.php?a_code=A-10-676-1&slc_lang=en&sid=1 Asma Mohammadi 100319475328460012602 100319475328460012602 No Abadan Faculty of Medical Sciences, Abadan, Iran. Ali Reza Balizadeh Karami 100319475328460012603 100319475328460012603 No Student Research Committee, Abadan Faculty of Medical Sciences, Abadan, Iran. Vahid Dehghan Mashtani 100319475328460012604 100319475328460012604 No Student Research Committee, Abadan Faculty of Medical Sciences, Abadan, Iran. Tooba Sahraei 100319475328460012605 100319475328460012605 No Abadan Faculty of Medical Sciences, Abadan, Iran. Zeinab Bandani Tarashoki 100319475328460012606 100319475328460012606 No Student Research Committee, Abadan Faculty of Medical Sciences, Abadan, Iran. Ehsan Khattavian 100319475328460012607 100319475328460012607 No : Student Research Committee, Abadan Faculty of Medical Sciences, Abadan, Iran. Sara Mobarak 100319475328460012608 100319475328460012608 No Abadan Faculty of Medical Sciences, Abadan, Iran. Hossein Moradi Kazerouni 100319475328460012609 100319475328460012609 No Student Research Committee, Abadan Faculty of Medical Sciences, Abadan, Iran. Esmat Radmanesh esmatradmanesh33@gmail.com. 100319475328460012610 100319475328460012610 Yes Abadan Faculty of Medical Sciences, Abadan, Iran & Student Research Committee, Abadan Faculty of Medical Sciences, Abadan, Iran.