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en Investigation of Decitabine Effects on HDAC3 and HDAC7 mRNA Expression in NALM-6 and HL-60 Cancer Cell Lines زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: Decitabine is a potent anticancer hypomethylating agent and changes the gene expression through the gene's promoter demethylation and also independently from DNA demethylation. So, the present study was designed to distinguish whether Decitabine, in addition to inhibitory effects on DNA methyltransferase, can change HDAC3 and HDAC7 mRNA expression in NALM-6 and HL-60 cancer cell lines. Methods: HL-60, NALM-6, and normal cells were cultured, and the Decitabine treatment dose was obtained (1 μM) through the MTT assay. Finally, HDAC3 and HDAC7 mRNA expression were measured by Real-Time PCR in HL-60 and NALM-6 cancerous cells before and after treatment. Furthermore, HDAC3 and HDAC7 mRNA expression in untreated HL-60 and NALM-6 cancerous cells were compared to normal cells.  Results: Our results revealed that the expression of HDAC3 and HDAC7 in HL-60 and NALM-6 cells increases as compared to normal cells. After treatment of HL-60 and NALM-6 cells with Decitabine, HDAC3, and HDAC7 mRNA expression were decreased significantly.  Conclusions: Our data confirmed that the effects of Decitabine are not limited to direct hypomethylation of DNMTs, but it can indirectly affect other epigenetic factors, such as HDACs activity, through converging pathways.</div> Decitabine, HDAC3, HDAC7, HL-60, NALM-6. 420 428 http://rbmb.net/browse.php?a_code=A-10-415-1&slc_lang=en&sid=1 Sina Dalvand sina.dalvand13@gmail.com. 100319475328460012542 100319475328460012542 Yes International Campus, Department of Biochemistry and Molecular Biology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Amin Namdari 100319475328460012543 100319475328460012543 No Department of Clinical Biochemistry, Fasa University of Medical Sciences, Fasa, Fars, Iran. Farzad Sepahvand 100319475328460012544 100319475328460012544 No Department of Clinical Biochemistry, Fasa University of Medical Sciences, Fasa, Fars, Iran. Mohammad Hassan Meshkibaf 100319475328460012545 100319475328460012545 No Department of Clinical Biochemistry, Fasa University of Medical Sciences, Fasa, Fars, Iran. GholamReza Ahmadpour 100319475328460012546 100319475328460012546 No Department of Medical Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.