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en The Impacts of Prepared Plasma-Activated Medium (PAM) Combined with Doxorubicin on the Viability of MCF-7 Breast Cancer Cells: A New Cancer Treatment Strategy بیوشیمی Biochemistry مقالات اصلی Original Article <div style="text-align: justify;">Background: For many years, the chemotherapeutic agent doxorubicin (DOX) has been used to treat various cancers; however, DOX initiates several critical adverse effects. Many studies have reported that non-thermal atmospheric pressure plasma can provide novel, but challenging, treatment strategies for cancer patients. To date, tissues and cells have been treated with plasma-activated medium (PAM) as a practical therapy. Consequently, due to the harmful adverse effects of DOX, we were motivated to elucidate the impact of PAM in the presence of DOX on MCF-7 cell proliferation. Methods: MTT assay, N-acetyl-L-cysteine (NAC) assay, and flow cytometry analysis were utilized in this research.  Results: The results demonstrated that 0.45 μM DOX combined with 3-min PAM significantly induced apoptosis (p< 0.01) through intracellular ROS generation in MCF-7 when compared with 0.45 μM DOX alone or 3-min PAM alone. In contrast, after treatment with 0.45 μM DOX plus 4-min PAM, cell necrosis was increased. Hence, DOX combined with 4-min PAM has cytotoxic effects with different mechanisms than 4-min PAM alone, in which the number of apoptotic cells increases. Conclusions: Although further investigations are crucial, low doses of DOX plus 3-min PAM could be a promising strategy for cancer therapy. The findings from this research may offer advantageous and innovative clinical strategies for cancer therapy using PAM.</div> Apoptosis, Breast cancer lymphedema, Doxorubicin, Plasma-activated medium (PAM), Necrosis. 640 652 http://rbmb.net/browse.php?a_code=A-10-763-1&slc_lang=en&sid=1 Setareh Zahedian 100319475328460012459 100319475328460012459 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Azadeh Hekmat hekmat@ut.ac.ir. 100319475328460012460 100319475328460012460 Yes Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Saeed Hesami Tackallou 100319475328460012461 100319475328460012461 No Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran. Mahmood Ghoranneviss 100319475328460012462 100319475328460012462 No Department of Plasma Physics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran.