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en Association of Exposure to Polycyclic Aromatic Hydrocarbons with Inflammation, Oxidative DNA Damage and Renal-pulmonary Dysfunctions in Barbecue Makers in Southern Nigeria بیوشیمی Biochemistry مقالات اصلی Original Article <div style="text-align: justify;">Background: Multiple organ dysfunctions have been linked to exposure to polycyclic aromatic hydrocarbons (PAH) and oxidative stress (OS), oxidative DNA damage, and inflammatory response to PAH have been implicated. The biomarkers of OS (malondialdehyde (MDA), total plasma peroxide (TPP), total antioxidant capacity (TAC), glutathione (GSH), nitric oxide (NO), oxidative stress index (OSI)); 8-hydroxy-2-deoxyguanosine (8-OHdG)); tumor necrosis factor-alpha (TNF-α)); 1-hydroxy pyrene (1-HOP)), serum and urine creatinine, uric acid (UA), estimated glomerular filtration rate (eGFR) and peak expiratory flow rate (PEFR) were assessed in barbecue makers. Methods: One hundred barbecue makers and 50 controls were enrolled into the study. Serum and urine creatinine, UA, TAC, MDA, GSH, NO and TPP were estimated by colorimetry, 8-OHdG and TNF-α by ELISA, PEFR using peak flow meter, 1-HOP by HPLC, eGFR and OSI by calculation. Results: Barbecue makers had lower TAC, PEFR, and higher TNF-α and OS compared to controls (P<0.05). Higher TNF-α, lipid peroxidation, and lower antioxidants were observed in barbecue makers who had worked for >5years compared to <5years (P<0.05). Increasing number of working hours was associated with higher NO, lipid peroxidation, OS and lower antioxidants in barbecue makers (P<0.05). Positive associations were observed between 1-HOP and TPP (r=0.570, P=0.000), OSI (r=0.299, P=0.035) and negative association between TAC and TNF-α (r=-0.209, P=0.037), MDA (r=-0.265, P=0.008) in barbecue makers. Conclusion: Increased lipid peroxidation, OS, inflammation and depressed antioxidants and lung function observed in barbecue makers suggest increased risk of chronic lung conditions which may be associated with exposure to PAH in barbecue fumes. </div> Inflammation, Kidney, Lipid Peroxidation, Lung, Oxidative Stress, Polycyclic Aromatic Hydrocarbon. 74 82 http://rbmb.net/browse.php?a_code=A-10-775-1&slc_lang=en&sid=1 Augusta Chinyere Nsonwu-Anyanwu austadechic@yahoo.com. 100319475328460012340 100319475328460012340 Yes Department of Medical Laboratory Science, University of Calabar, PMB 1115, Calabar, Nigeria. Augusta Ndudi Idenyi 100319475328460012341 100319475328460012341 No Department of Medical Laboratory Science, University of Calabar, PMB 1115, Calabar, Nigeria. Sunday Jeremiah Offor 100319475328460012342 100319475328460012342 No Department of Medical Laboratory Science, University of Calabar, PMB 1115, Calabar, Nigeria. Caroline Chinenyenwa Thomas 100319475328460012343 100319475328460012343 No Department of Medical Laboratory Science, University of Calabar, PMB 1115, Calabar, Nigeria. Friday Okputu 100319475328460012344 100319475328460012344 No Department of Medical Laboratory Science, University of Calabar, PMB 1115, Calabar, Nigeria. Clement Emmanuel Edet 100319475328460012345 100319475328460012345 No Department of Medical Laboratory Science, University of Calabar, PMB 1115, Calabar, Nigeria. Chinyere Adanna Opara Usoro 100319475328460012346 100319475328460012346 No Department of Medical Laboratory Science, University of Calabar, PMB 1115, Calabar, Nigeria.