Reports of Biochemistry and Molecular Biology
rbmb.net
Basic Sciences
http://rbmb.net
1
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2322-3480
2322-3480
10.61186/rbmb
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jalali
1395
2
1
gregorian
2016
5
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online
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fulltext
en
Comparative In silico Study of Sex-Determining Region Y (SRY) Protein Sequences Involved in Sex-Determining
زیست شناسی ملکولی
Molecular Biology
<p style="text-align: justify;"><em><strong>Background:</strong></em> The SRY gene <em>(SRY)</em> provides instructions for making a transcription factor called the sex-determining region Y protein. The sex-determining region Y protein causes a fetus to develop as a male. In this study, SRY of 15 spices included of human, chimpanzee, dog, pig, rat, cattle, buffalo, goat, sheep, horse, zebra, frog, urial, dolphin and killer whale were used for determine of bioinformatic differences.</p>
<p style="text-align: justify;"><em><strong>Methods:</strong></em> Nucleotide sequences of<em> SRY</em> were retrieved from the NCBI databank. Bioinformatic analysis of <em>SRY</em> is done by CLC Main Workbench version 5.5 and ClustalW (http:/www.ebi.ac.uk/clustalw/) and MEGA6 softwares.</p>
<p style="text-align: justify;"><em><strong>Results:</strong></em> The multiple sequence alignment results indicated that <em>SRY</em> protein sequences from Orcinus orca (killer whale) and <em>Tursiopsaduncus</em> (dolphin) have least genetic distance of 0.33 in these 15 species and are 99.67% identical at the amino acid level. <em>Homosapiens</em> and <em>Pantroglodytes </em>(chimpanzee) have the next lowest genetic distance of 1.35 and are 98.65% identical at the amino acid level.</p>
<p style="text-align: justify;"><em><strong>Conclusion:</strong></em> These findings indicate that the SRY proteins are conserved in the 15 species, and their evolutionary relationships are similar.</p>
Bioinformatics analysis, SRY gene, Phylogeny
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81
http://rbmb.net/browse.php?a_code=A-10-1-53&slc_lang=en&sid=1
Masoume
Vakili Azghandi
10031947532846006126
10031947532846006126
No
Department of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
Mohammadreza
Nasiri
nassiryr@um.ac.ir
10031947532846006127
10031947532846006127
Yes
Department of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
Ali
Shamsa
10031947532846006128
10031947532846006128
No
Department of Urology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Mohsen
Jalali
10031947532846006129
10031947532846006129
No
Department of Urology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Mohammad Mahdi
Shariati
10031947532846006130
10031947532846006130
No
Department of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran