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Reports of Biochemistry and Molecular Biology
rbmb.net
Basic Sciences
http://rbmb.net
1
admin
2322-3480
2322-3480
10.61882/rbmb
en
jalali
1395
2
1
gregorian
2016
5
1
4
2
online
1
fulltext
en
Construction of a Novel DNA Vaccine Candidate Encoding an HspX-PPE44-EsxV Fusion Antigen of Mycobacterium tuberculosis
زیست شناسی ملکولی
Molecular Biology
<p style="text-align: justify;"><em><strong>Background:</strong></em> <em>Mycobacterium tuberculosis</em> is the causative agent of tuberculosis (TB). <em>Bacille Calmette-Guerin</em> (BCG) vaccine, is not effective in adults, therefore, many efforts have been made to produce an effective adult TB vaccine. The aim of this study was to develop a new tuberculosis DNA vaccine candidate encoding a recombinant HspX-PPE44-EsxV fusion antigen of <em>M. tuberculosis. </em></p>
<p style="text-align: justify;"><em><strong>Methods:</strong></em> A fusion DNA segment consisting of HspX, linker, PPE44, linker, and EsxV, after codon optimization, was designed. The fusion DNA was cloned and its sequence confirmed. Then, expression of a recombinant pcDNA3.1 (+)/HspX-PPE44-EsxV plasmid in Chinese hamster ovary (CHO) cells was verified by RT-PCR and Western-blot analysis.</p>
<p style="text-align: justify;"><em><strong>Results:</strong></em> A 1968 bp band in RT-PCR and a 68 kDa band on Western-blot analysis confirmed transcription and expression of recombinant <em>hspX-ppe44-esxV</em> in eukaryotic cells.</p>
<p style="text-align: justify;"><em><strong>Conclusions: </strong></em>A recombinant DNA segment encoding the HspX-PPE44-EsxV fusion antigen of <em>M. tuberculosis</em> was constructed and considered to be tested as a new TB DNA vaccine candidate.</p>
DNA vaccine, EsX, Hsp, Mycobacterium tuberculosis, PPE44
89
97
http://rbmb.net/browse.php?a_code=A-10-1-55&slc_lang=en&sid=1
Bagher
Moradi
10031947532846006137
10031947532846006137
No
Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Mojtaba
Sankian
10031947532846006138
10031947532846006138
No
Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Yousef
Amini
10031947532846006139
10031947532846006139
No
Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Zahra
Meshkat
meshkatz@mums.ac.ir
10031947532846006140
10031947532846006140
Yes
Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran