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en A New Method to Estimate Inhibition Percentage of Endogenous Digitalis in Patients with Pre-eclampsia بیوشیمی Biochemistry مقالات اصلی Original Article <div style="text-align: justify;">Background: Pre-eclampsia is an idiopathic pregnancy disorder characterized by appearance proteinuria and hypertension, with poorly understood etiology. It has been linked to a variety of system abnormalities, including ion transport disorders in neonatal, maternal, and placental cell lines. A new method was described to evaluate the inhibition percentage of endogenous digitalis in plasma of pre-eclampsia patients compared with normal pregnancies, with the estimation of sensitivity and specificity of the proposed test. Methods: This was a case-control study consisting of 130 cases that were divided into three groups, 55 normal pregnancies (positive control), 30 non-pregnant women (negative control), and 45 preeclampsia (patients). The new method included the estimation of the percentage inhibition of endogenous digitalis by measuring specific enzyme activity of Na-K ATPase for the patient and positive control. The results were analyzed using the statistical package for social sciences (SPSS®) software version 26.0. A p-value of≤ 0.05 was considered significant. Results: In the pre-eclampsia patient, the specific activity of Na-K ATPase was significantly lower with mean= 0.239 mg/g±0.043 compared to the control group which was 0.397 mg/g±0.021, p<0.001. While the result of inhibition percentage of endogenous digitalis showed significantly higher in the pre-eclampsia patient (mean= 35.852 mg/g %±2.692%) compared to the control group (mean=17.964%±1.784), with a p< 0.001. Conclusions: Pre-eclampsia is linked with lower erythrocyte sodium pump activity significantly in pre-eclampsia patients than in normal pregnancies. Also, results show the inhibited percentage of endogenous digitalis elevation in patients with pre-eclampsia compared with normal pregnancy.</div> Endogenous Digitalis, Hypertension, Inhibition Percentage, Pre-eclampsia. 138 145 http://rbmb.net/browse.php?a_code=A-10-872-1&slc_lang=en&sid=1 Hawraa Saad Al-Kawaz hawraa.saad@mustaqbal-college.edu.iq. 100319475328460012366 100319475328460012366 Yes Department of Medical Laboratories Techniques, Al-Mustaqbal University College, Babylon, Iraq. Oda Mizil Yasser 100319475328460012367 100319475328460012367 No Department of Chemistry, College of Science, University of Babylon, Babylon. Mazin Jaafar Mousa 100319475328460012368 100319475328460012368 No College of Pharmacy, University of Babylon, Babylon, Iraq.