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'); Reports of Biochemistry and Molecular Biology rbmb.net Basic Sciences http://rbmb.net 1 admin 2322-3480 2322-3480 10.61882/rbmb en jalali 1395 7 1 gregorian 2016 10 1 5 1 online 1 fulltext
en Evaluation of SCD and FASN Gene Expression in Baluchi, Iran-Black, and Arman Sheep زیست شناسی ملکولی Molecular Biology <p style="text-align: justify;"><em><strong>Background:</strong></em> With the increasing concern for health and nutrition, dietary fat has attracted considerable attention. The composition of fatty acids in the diet is important because they are associated with major diseases including cancers, diabetes, and cardiovascular disease. The fatty acid synthase (FASN) and stearoyl-CoA desaturase (delta-9-desaturase) (SCD) genes affect fatty acid composition (1). The expression of SCD and FASN genes is related to an increase in conjugated linoleic acid (CLA) in dairy products, which benefits human health.The aim of current study was to investigate expression changes of SCD and FASN genes that resulted from crossbreeding the local Baluchi sheep with alien breeds.</p> <p style="text-align: justify;"><strong><em>Methods:</em></strong> We collected tissue samples from the mammary glands of 24 single-born ewes from local Baluchi and synthetic Iran-Black and Arman sheep breeds in the Abbas Abad breeding center. After RNA extraction and cDNA synthesis, real-time PCR was performed with all samples in triplicate.</p> <p style="text-align: justify;"><em><strong>Results:</strong></em>The maximum and minimum expression of SCD and FASN genes was in the local Baluchi sheep and the crossbred Arman sheep, respectively.</p> <p style="text-align: justify;"><em><strong>Conclusions:</strong></em> With the highest SCD and FASN gene expression in local Baluchi sheep and relatively less expression of these genes in synthetic Iran-Black and Arman Sheep breeds, it may be necessary to consider the consequences of crossbreeding local sheep and the fatty acid composition of their dairy products.</p> SCD, FASN ,Gene expression, Real-time PCR 33 39 http://rbmb.net/browse.php?a_code=A-10-1-62&slc_lang=en&sid=1 Mohammad Salmani Izadi 10031947532846006167 10031947532846006167 No Department of Animal Sciences, Ferdowsi University of Mashhad, International Campus, Mashhad, Iran Abbas Ali Naserian abasalin@yahoo.com 10031947532846006168 10031947532846006168 Yes Department of Animal Sciences, Ferdowsi University of Mashhad, Mashhad, Iran. Mohammad Reza Nasiri 10031947532846006169 10031947532846006169 No Department of Animal Sciences, Ferdowsi University of Mashhad, Mashhad, Iran. Reza Majidzadeh Heravi 10031947532846006170 10031947532846006170 No Department of Animal Sciences, Ferdowsi University of Mashhad, Mashhad, Iran. Reza Valizadeh 10031947532846006171 10031947532846006171 No Department of Animal Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.