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en Regulation of Focal Adhesion Dynamics and Cell Migration by PLC/PI3K-Mediated Metabolism of PtdIns (4,5) P2 in a Breast Cancer Cell Line زیست شناسی سلولی Cell Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: Focal adhesions (FAs) are highly dynamic complex structures that assembled and disassembled on an ongoing basis. The balance between the two processes mediates various aspects of cell behavior, ranging from cell adhesion to cell migration. Assembly and disassembly processes of FAs are regulated by a variety of cellular signaling proteins and adaptors. We previously demonstrated that local levels of Phosphatidylinositol 4,5‐bisphosphate (PtdIns(4,5)P2) in MDA-MB-231 cells increases during FA assembly and declines during disassembly. In this study we aimed to investigate whether PtdIns(4,5)P2 regulates FA turnover. Methods: MDA-MB-231 cells were co-transfected with a labeling vinculin (or zyxin) and the PLC𝛅1-PH biosensor to visualize FA localization and PtdIns(4,5)P2 in the cell membrane. We also used pharmacological inhibitors to determine the mechanism underlying the changes of PtdIns(4,5)P2 level during FA turnover and cell migration. Immunostaining, immunoprecipitation, and Western blotting were used to examine the localization and interaction between phospholipase C (PLC)/phosphatidylinositol 3-kinase (PI3K) FA proteins. Results: We showed that inhibition of PLC, PI3K significantly reduced the decline of PtdIns(4,5)P2 levels within FA disassembly and the slowdown rate of FA turnover and cell migration. We also showed that the inhibition of enzymes implicated in the downstream pathway of PtdIns(4,5)P2, such as diacylglycerol kinase (DAGK) and protein kinase C (PKC) significantly reduced FA turnover time and the speed of cell migration. Additionally, we demonstrated that PLC but not PI3K interact with FAs. In conclusion, Conclusions: This study suggests that dynamical changes of PtdIns(4,5)P2 might regulate FA turnover and facilitate cell migration.</div> Cell Migration, Focal Adhesion Turnover, MDA-MB-231 Breast Cancer Cell Line, Ptdins(4,5)P2, PLC, PI3K. 270 281 http://rbmb.net/browse.php?a_code=A-10-749-6&slc_lang=en&sid=1 Dhurgham Al-Fahad dhurgham.alfahad@sci.utq.edu.iq 100319475328460012213 100319475328460012213 Yes Department of Pharmaceutical Sciences, College of Pharmacy University of Thi-Qar, Iraq Firas Alyaseen 100319475328460012214 100319475328460012214 No Department of Pharmaceutical Sciences, College of Pharmacy University of Thi-Qar, Iraq. Ahmed Al-Amery 100319475328460012215 100319475328460012215 No Faculty of Education, Soran University, Erbil, Kurdistan Region, Iraq. Clementino Ibeas Bin 100319475328460012216 100319475328460012216 No Ilumix Biotech Ltd, London, United Kingdom.