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Reports of Biochemistry and Molecular Biology
rbmb.net
Basic Sciences
http://rbmb.net
1
admin
2322-3480
2322-3480
10.61882/rbmb
en
jalali
1401
8
1
gregorian
2022
11
1
11
3
online
1
fulltext
en
Association Between HOTAIR rs920778 and H19 rs3741219 Polymorphisms with Hashimoto’s Thyroiditis (HT) and Graves’ Disease (GD)
زیست شناسی ملکولی
Molecular Biology
مقالات اصلی
Original Article
<div style="text-align: justify;"><span style="font-size:12pt"><span style="font-family:TimesNewRomanPS-BoldItalicMT"><span style="color:#000000"><span style="font-weight:bold"><span style="font-style:italic">Background: </span></span></span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">Graves’ disease (GD) and Hashimoto’s thyroiditis (HT) are two autoimmune thyroid </span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">diseases (AITDs). The current study aimed to assess possible association between HOTAIR rs920778 </span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">and H19 rs3741219 polymorphisms with GD and HT.</span></span></span><br>
</div>
<div style="text-align: justify;"><span style="font-size:12pt"><span style="font-family:TimesNewRomanPS-BoldItalicMT"><span style="color:#000000"><span style="font-weight:bold"><span style="font-style:italic">Methods: </span></span></span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">We recruited 248 patients with autoimmune thyroid disease (133 HT patients and 115 GD </span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">patients) and 135 age- and sex-matched controls. The PCR-RFLP method was applied for genotyping </span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">of HOTAIR rs920778, and H19 rs3741219 polymorphisms.</span></span></span><br>
</div>
<div style="text-align: justify;"><span style="font-size:12pt"><span style="font-family:TimesNewRomanPS-BoldItalicMT"><span style="color:#000000"><span style="font-weight:bold"><span style="font-style:italic">Results: </span></span></span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">The HOTAIR rs920778 GA frequency was significantly higher in control compared to HT </span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">group. The Overdominant model showed a significant association with the risk of HT. However, no </span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">significant association was observed between this polymorphism and HT susceptibility in dominant </span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">and recessive models. The H19 rs3741219 GA was more repeated in HT patients compared to control </span></span></span></div>
<div style="text-align: justify;"><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">group, but the difference was not significant. There was no association between HOTAIR rs920778 </span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">and H19 rs3741219 polymorphisms with GD in all genetic models.</span></span></span><br>
</div>
<div style="text-align: justify;"><span style="font-size:12pt"><span style="font-family:TimesNewRomanPS-BoldItalicMT"><span style="color:#000000"><span style="font-weight:bold"><span style="font-style:italic">Conclusions:</span></span></span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">Our findings indicated that HOTAIR rs920778 polymorphism decreased the risk of HT. </span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">Since, this the first study, further studies with different races are required to confirm our results. </span></span></span></div>
<div style="text-align: justify;"></div>
Hashimoto’s thyroiditis, Graves’ disease, HOTAIR, H19, Polymorphism
377
385
http://rbmb.net/browse.php?a_code=A-10-938-2&slc_lang=en&sid=1
Alireza
Beiramzadeh
100319475328460013363
100319475328460013363
No
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
Zahra
Heidari
100319475328460013364
100319475328460013364
No
Department of Internal Medicine, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Mahtab
Norozi
100319475328460013365
100319475328460013365
No
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Mohsen
Saravani
moh.saravani@gmail.com
100319475328460013366
100319475328460013366
Yes
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran & Cellular and Molecular Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan,Iran.