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'); Reports of Biochemistry and Molecular Biology rbmb.net Basic Sciences http://rbmb.net 1 admin 2322-3480 2322-3480 10.61882/rbmb en jalali 1401 8 1 gregorian 2022 11 1 11 3 online 1 fulltext
en Anti-RAGE (Receptor Advanced Glycation End products) Antibody Improves Diabetic Retinopathy in Rats via Hypoglycemic and Anti-inflammatory Mechanism زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="font-family:TimesNewRomanPS-BoldItalicMT"><span style="color:#000000"><span style="font-weight:bold"><span style="font-style:italic">Background: </span></span></span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">Receptor advanced glycation end products (RAGE) activation plays an essential role&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">in diabetic retinopathy (DR) progression. This study was aimed to explore the role of anti-RAGE&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">antibodies (RAGE antagonists) in inhibiting DR progression through their hypoglycemic and anti&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">inflammatory mechanism in diabetic retinopathy induced rats.</span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:12pt"><span style="font-family:TimesNewRomanPS-BoldItalicMT"><span style="color:#000000"><span style="font-weight:bold"><span style="font-style:italic">Methods: </span></span></span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">A total of 30 male Wistar rats were randomly divided into five group. The group was&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">consisted of normal control group, DR group without treatment, DR group with anti-RAGE 1 </span></span></span><span style="font-size:12pt"><span style="font-family:Symbol"><span style="color:#000000"></span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">g/kg&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">BW, 10 </span></span></span><span style="font-size:12pt"><span style="font-family:Symbol"><span style="color:#000000"></span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">g/kg BW, and 100 </span></span></span><span style="font-size:12pt"><span style="font-family:Symbol"><span style="color:#000000"></span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">g/kg BW. To assess the diabetic retinopathy, fundus photographs were&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">taken every week using a camera with 16x magnification placed in front of the rat&#39;s eyes. Blood&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">glucose was checked by the glucose oxidase-peroxidase method. Retinal TNF-</span></span></span><span style="font-size:12pt"><span style="font-family:Symbol"><span style="color:#000000"> </span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">levels and VEGF&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">were examined using an enzyme-linked immunosorbent assay (ELISA) kit.</span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:12pt"><span style="font-family:TimesNewRomanPS-BoldItalicMT"><span style="color:#000000"><span style="font-weight:bold"><span style="font-style:italic">Results: </span></span></span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">The finding of this study showed that anti-RAGE treatment at dose of 10 and 100 </span></span></span><span style="font-size:12pt"><span style="font-family:Symbol"><span style="color:#000000"></span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">g/kg&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">BW, HbA1c levels were significantly higher (p< 0.05) compared to the normal control group but&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">significantly lower (p< 0.05) than in the diabetes group. The mean blood vessel diameter in the&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">DR+anti-RAGE 10 and 100 </span></span></span><span style="font-size:12pt"><span style="font-family:Symbol"><span style="color:#000000"></span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">g/kg BW groups was significantly lower than in the diabetic retinopathy&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">group (p< 0.05). The administration of anti-RAGE 10 and 100 </span></span></span><span style="font-size:12pt"><span style="font-family:Symbol"><span style="color:#000000"></span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">g/kg BW showed the ability to&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">significantly reduce VEGF levels compared to the DR group (p< 0.05).</span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:12pt"><span style="font-family:TimesNewRomanPS-BoldItalicMT"><span style="color:#000000"><span style="font-weight:bold"><span style="font-style:italic">Conclusions:</span></span></span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">This study revealed at doses of 10 and 100 </span></span></span><span style="font-size:12pt"><span style="font-family:Symbol"><span style="color:#000000"></span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">g/kg BW, anti-RAGE antibodies improved&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">diabetic retinopathy in Wistar rats through hypoglycemic effects and anti-inflammatory mechanisms.</span></span></span></div> <div style="text-align: justify;"></div> Anti-RAGE (Receptor Advanced Glycation End products), Diabetic Retinopathy, Glycated Hemoglobin A, Hypoglycemic Agents, Peroxidases, Vascular Endothelial Growth Factor A 394 399 http://rbmb.net/browse.php?a_code=A-10-988-1&slc_lang=en&sid=1 Ramzi Amin ramziamin20@gmail.com. 100319475328460013373 100319475328460013373 Yes Department of Ophthalmology, Faculty of Medicine Universitas Sriwijaya/ Dr Moh Hoesin General Hospital, Palembang, Indonesia Tiara Bunga Indiarsih 100319475328460013374 100319475328460013374 No Specialized Residency Training, Faculty of Medicine Universitas Sriwijaya/Dr Moh Hoesin General Hospital, Palembang, Indonesia. Prima Maya Sari 100319475328460013375 100319475328460013375 No Department of Ophthalmology, Faculty of Medicine Universitas Sriwijaya/ Dr Moh Hoesin General Hospital, Palembang, Indonesia. Petty Purwanita 100319475328460013376 100319475328460013376 No Department of Ophthalmology, Faculty of Medicine Universitas Sriwijaya/ Dr Moh Hoesin General Hospital, Palembang, Indonesia.