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en Protective Effects of Cinnamic Acid Against Hyperglycemia Induced Oxidative Stress and Inflammation in HepG2 Cells بیوشیمی Biochemistry مقالات اصلی Original Article <div style="text-align: justify;">Background: Cinnamic acid, a phenylpropanoid acid, has been investigated as a potential alternative therapy for diabetes and its complications in some studies.  Methods: In the first stage, the viability of HepG2 cells at different concentrations of glucose and CA was assessed by MTT assay. Oxidative stress markers) CAT, GPx, GSH, and MDA) were measured spectrophotometrically. After RNA extraction, the effect of different concentrations of CA on the expression of DPP4 and inflammatory factors (IL-6, NF- κB) in HepG2 cells was assessed using real-time PCR. Results: In HepG2 cells, CA increased catalase and glutathione peroxidase activity and GSH production in a dose-dependent manner in the presence of high glucose concentrations, with the greatest effect seen at a concentration of 75 mg/ml. Also, it reduced the amount of MDA in high-glucose HepG2 cells. Furthermore, CA decreased the expression of DPP4, NF- κB, and IL-6 genes in HepG2 cells in the presence of high glucose levels. Conclusions: The results of our study indicated that CA reduced hyperglycemia-induced complications in HepG2 cells by decreasing inflammatory gene expression, including IL-6 and NF- κB and inhibiting the expression of DPP4, and limiting oxidative stress.</div> Cinnamic acid, Diabetes, HepG2 cells, Hyperglycemia ss, Oxidative stre. 1 12 http://rbmb.net/browse.php?a_code=A-10-990-1&slc_lang=en&sid=1 Mohammad Yazdi 100319475328460017783 100319475328460017783 No Department of Biochemistry, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran & Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran. Amirhossein Nafari 100319475328460017784 100319475328460017784 No Student Research Committee, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran & Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran. Mojgan Azadpour 100319475328460017785 100319475328460017785 No Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran. Mahdi Alaee 100319475328460017786 100319475328460017786 No Department of Biochemistry, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. Forouzan Hadipour Moradi 100319475328460017787 100319475328460017787 No Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran. Razieh Choghakhori 100319475328460017788 100319475328460017788 No Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran. Maryam Hormozi 100319475328460017789 100319475328460017789 No Department of Biochemistry, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran & Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran. Hassan Ahmadvand h.ahmadvand@umsha.ac.ir 100319475328460017790 100319475328460017790 Yes Department of Biochemistry, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran & Medicinal plants and natural products research center, Hamadan university of medical sciences, Hamadan, Iran.