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en Synergistic Anti-Cancer Activity of the Combination of 1,25-Dihydroxyvitamin D3 and Retinoic Acid in U937 Cell Line زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: MicroRNA is a form of non-coding RNAs that able to regulate gene expression. miR-424 is one of the members of the regulatory family, which plays an important role in the proliferation and differentiation of myeloid cells. Epigenetic changes can change the level of miR-424 under environmental factors. Therefore, the level of expression of miR-424 in U937 cells of the myeloid line was evaluated in this research under the influence of vitamin D3 (VitD3) and retinoic acid (RA). Methods: In this study, U937 cells were cultured in the presence of VitD3, and RA to evaluate cell proliferation, viability via the trypan blue exclusion test, and expression level of miR-424 by real-time PCR at specific times. Results: Cell proliferation has shown a significant decrease in the RA group versus other groups during incubation times (P < 0.05). In VitD3 group, there was a significant increase in cell proliferation after 24- and 48-hours incubation periods versus other groups. In the VitD3 and RA groups, the increase of cell proliferation caused the downregulation of miR-424. In addition, the upregulation of VitD3 group and downregulation of the RA group were significant versus the control group (P < 0.05). Conclusions: We concluded that the expression level of miR-424 was critically affected in the dose- and time-dependent of RA and VitD3 treatment in the U937 cell line. Treatment with VitD3 decreased the expression of miR-424 and RA treatment increase miR-424 expression level in physiological doses.</div> Keywords: Cell Proliferation, Differentiation, miR-424, U937 Cells. 440 449 http://rbmb.net/browse.php?a_code=A-10-994-1&slc_lang=en&sid=1 Abdollah Davodian 100319475328460016721 100319475328460016721 No Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Kobra Foroughi 100319475328460016722 100319475328460016722 No Department of Medical Biotechnology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran. Amir Atashi 100319475328460016723 100319475328460016723 No Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, Iran. Masoud Soleimani soleim_m@modares.ac.ir 100319475328460016724 100319475328460016724 Yes Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran & Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.