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Aly Hassan E, Mohamed Elsaid A, Mahmoud El-Refaey A, Abou Elzahab M, Mahfouz Youssef M, Elmougy R. Association of ABCB1(Rs10276036, C/T) Gene, IL-18, and TNFα as Risk Factors for Nephrotic Syndrome Incidence. rbmb.net 2024; 13 (1) :67-78
URL: http://rbmb.net/article-1-1356-en.html
URL: http://rbmb.net/article-1-1356-en.html
Eglal Aly Hassan 
, Afaf Mohamed Elsaid , Ahmed Mahmoud El-Refaey , Mohammed Abou Elzahab , Magdy Mahfouz Youssef , Rehab Elmougy *
, Afaf Mohamed Elsaid , Ahmed Mahmoud El-Refaey , Mohammed Abou Elzahab , Magdy Mahfouz Youssef , Rehab Elmougy *
Chemistry Department, Faculty of Science, Mansoura University, Mansoura 35516, Egypt.
Abstract: (475 Views)
Background: The most common cause of Nephrotic Syndrome (NS) in children is idiopathic NS, also called nephrosis. The most prominent clinical signs are hyperlipidemia, severe proteinuria, edema, swelling of body tissues, and an increased risk of infection. The object of this study was to examine the correlation of the ABCB1 gene (rs10276036, C > T), IL-18, and TNFα to the prevalence of NS among Egyptian children having NS.
Methods: This study included 100 participants with NS and 100 healthy controls. To analyze the ABCB1 gene (rs10276036 C >T) variant PCR technique was used. IL-18 and TNF levels were estimated using Enzyme-Linked Immunosorbent Assay (ELISA).
Results: Increased frequency of CT and TT genotypes of the ABCB1 gene (rs10276036 C / T) in NS patients compared to controls, with p-value = 0.001, OR = 2.270, CI = (1.550-3.327) for CT genotype and p-value = 0.001, OR = 5.070, CI = (2.463-10.438) for TT genotype. The frequencies of ABCB1 (rs10276036 C >T) genotypes were statistically significant in the dominant model (OR 2.560; p< 0.001) and in the recessive model OR, 3.231; p= 0.001). Significantly high levels of both IL-18 and TNFα were found in NS patients compared to controls.
Conclusion: The ABCB1gene (rs10276036 C/T), IL-18, and TNFα are associated with the prevalence of NS in Egyptian children and might be considered as independent risk factors for its incidence.
Methods: This study included 100 participants with NS and 100 healthy controls. To analyze the ABCB1 gene (rs10276036 C >T) variant PCR technique was used. IL-18 and TNF levels were estimated using Enzyme-Linked Immunosorbent Assay (ELISA).
Results: Increased frequency of CT and TT genotypes of the ABCB1 gene (rs10276036 C / T) in NS patients compared to controls, with p-value = 0.001, OR = 2.270, CI = (1.550-3.327) for CT genotype and p-value = 0.001, OR = 5.070, CI = (2.463-10.438) for TT genotype. The frequencies of ABCB1 (rs10276036 C >T) genotypes were statistically significant in the dominant model (OR 2.560; p< 0.001) and in the recessive model OR, 3.231; p= 0.001). Significantly high levels of both IL-18 and TNFα were found in NS patients compared to controls.
Conclusion: The ABCB1gene (rs10276036 C/T), IL-18, and TNFα are associated with the prevalence of NS in Egyptian children and might be considered as independent risk factors for its incidence.
Keywords: ATP Binding Cassette Transporter, Interleukin-18, Polymerase Chain Reaction, Subfamily B, Tumor Necrosis Factor-alpha.
Type of Article: Original Article |
Subject:
Molecular Biology
Received: 2024/03/1 | Accepted: 2024/07/7 | Published: 2024/10/22
Received: 2024/03/1 | Accepted: 2024/07/7 | Published: 2024/10/22
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