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Subali D, Kurnia G, Yanti Y, Christos R E, Shih Y. Unveiling Neuroprotective Potential in Tempeh Peptide Extracts by In Vitro Screening of Anti-Alzheimer’s Compounds. rbmb.net 2025; 14 (1) :46-56
URL: http://rbmb.net/article-1-1392-en.html
URL: http://rbmb.net/article-1-1392-en.html
Department of Biotechnology, Faculty of Bioscience, Technology, and Innovation, Atma Jaya Catholic University of Indonesia, Jakarta 12930, Indonesia.
Abstract: (843 Views)
Background: Alzheimer’s Disease (AD) incidence and prevalence increase every year, commonly related to neuron inflammation and degeneration conditions. Tempeh, a traditional fermented product from Indonesia, was reported to have anti-inflammatory, antioxidant, and anti-Alzheimer properties. However, anti-Alzheimer properties of tempeh peptide extracts have not been extensively examined. This research studied the effect of the extracted peptide from tempeh in preventing and delaying Alzheimer’s disease.
Methods: Tempeh peptide was extracted using water maceration and quantified using HPLC and spectrophotometry. Anti-Alzheimer properties of tempeh were analyzed with Ellman’s assay of anticholinesterase and in vitro gene expression analysis using LPS-induced neural Schwann cells.
Results: As a result, tempeh contained 19.27% of GABA, which is reported to have anti-Alzheimer properties, and other amino acids. Tempeh peptide extract at 12.5 µg/mL had strong inhibition activity toward acetylcholinesterase at 12.61%, and 100 µg/mL of tempeh peptide extract had 8.97% butyrylcholinesterase inhibition activity. Tempeh peptides extract also altered the expression of various genes related to Alzheimer’s disease, such as TNF-α, BACE 1, Ntrk 1, BDNF 2, and APP.
Conclusion: This research proved that various peptides from tempeh have anti-Alzheimer properties.
Keywords: Alzheimer disease, Gene expression, Peptides, Soy Foods.
Methods: Tempeh peptide was extracted using water maceration and quantified using HPLC and spectrophotometry. Anti-Alzheimer properties of tempeh were analyzed with Ellman’s assay of anticholinesterase and in vitro gene expression analysis using LPS-induced neural Schwann cells.
Results: As a result, tempeh contained 19.27% of GABA, which is reported to have anti-Alzheimer properties, and other amino acids. Tempeh peptide extract at 12.5 µg/mL had strong inhibition activity toward acetylcholinesterase at 12.61%, and 100 µg/mL of tempeh peptide extract had 8.97% butyrylcholinesterase inhibition activity. Tempeh peptides extract also altered the expression of various genes related to Alzheimer’s disease, such as TNF-α, BACE 1, Ntrk 1, BDNF 2, and APP.
Conclusion: This research proved that various peptides from tempeh have anti-Alzheimer properties.
Keywords: Alzheimer disease, Gene expression, Peptides, Soy Foods.
Background: Alzheimer’s Disease (AD) incidence and prevalence increase every year, commonly related to neuron inflammation and degeneration conditions. Tempeh, a traditional fermented product from Indonesia, was reported to have anti-inflammatory, antioxidant, and anti-Alzheimer properties. However, anti-Alzheimer properties of tempeh peptide extracts have not been extensively examined. This research studied the effect of the extracted peptide from tempeh in preventing and delaying Alzheimer’s disease.
Methods: Tempeh peptide was extracted using water maceration and quantified using HPLC and spectrophotometry. Anti-Alzheimer properties of tempeh were analyzed with Ellman’s assay of anticholinesterase and in vitro gene expression analysis using LPS-induced neural Schwann cells.
Results: As a result, tempeh contained 19.27% of GABA, which is reported to have anti-Alzheimer properties, and other amino acids. Tempeh peptide extract at 12.5 µg/mL had strong inhibition activity toward acetylcholinesterase at 12.61%, and 100 µg/mL of tempeh peptide extract had 8.97% butyrylcholinesterase inhibition activity. Tempeh peptides extract also altered the expression of various genes related to Alzheimer’s disease, such as TNF-α, BACE 1, Ntrk 1, BDNF 2, and APP.
Conclusion: This research proved that various peptides from tempeh have anti-Alzheimer properties.
Methods: Tempeh peptide was extracted using water maceration and quantified using HPLC and spectrophotometry. Anti-Alzheimer properties of tempeh were analyzed with Ellman’s assay of anticholinesterase and in vitro gene expression analysis using LPS-induced neural Schwann cells.
Results: As a result, tempeh contained 19.27% of GABA, which is reported to have anti-Alzheimer properties, and other amino acids. Tempeh peptide extract at 12.5 µg/mL had strong inhibition activity toward acetylcholinesterase at 12.61%, and 100 µg/mL of tempeh peptide extract had 8.97% butyrylcholinesterase inhibition activity. Tempeh peptides extract also altered the expression of various genes related to Alzheimer’s disease, such as TNF-α, BACE 1, Ntrk 1, BDNF 2, and APP.
Conclusion: This research proved that various peptides from tempeh have anti-Alzheimer properties.
Type of Article: Original Article |
Subject:
Molecular Biology
Received: 2024/05/18 | Accepted: 2025/09/13 | Published: 2025/12/9
Received: 2024/05/18 | Accepted: 2025/09/13 | Published: 2025/12/9
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