Volume 6, Issue 1 (Vol.6 No.1 Oct 2017)                   rbmb.net 2017, 6(1): 66-73 | Back to browse issues page

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Toghraie F S, Sharifzadeh S M, Ramezani A, Mahmoudi Maymand E, Yazdanpanah-Samani M, Ghaderi A. Cloning and Expression of Recombinant Human Interleukin-7 in Chinese Hamster Ovary (CHO) Cells. rbmb.net 2017; 6 (1) :66-73
URL: http://rbmb.net/article-1-151-en.html
Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical science, Shiraz, Iran - Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Abstract:   (7234 Views)

Background: The critical role of interleukin-7 (IL-7) in homeostatic proliferation and T cell survival has made it a promising cytokine for the treatment of various clinical conditions, especially those associated with lymphopenia.

Methods: In the present study we expressed recombinant human interleukin-7 (rhIL-7) in Chinese hamster ovary (CHO)-K1 cells. CHO-K1 cells were stably transfected with both circular and linear forms of the pBud-hIL-7 recombinant by electroporation. Expression of rhIL-7 in CHO-K1 cells was confirmed by enzyme-linked immunosorbent assay (ELISA) and dot and western blots.

Results: On western blots of transformed cells, a single 25 kDa band was observed, consistent with the expected molecular weight of glycosylated hIL-7. No significant expression difference was observed between cells transfected with circular or linear plasmids.

Conclusions: We established a stable CHO-K1 cell line expressing rhIL-7, which we consider to be a promising system for the production of rhIL-7 as a biopharmaceutical.

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Type of Article: Original Article | Subject: Special
Received: 2017/03/15 | Accepted: 2017/03/15 | Published: 2017/03/15

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