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Savari F, Mard S A, Rezaie A, Kalantar M. Effect of Zinc Oxide Nanoparticles on Hepatic Ischemia-Reperfusion Injury: Role of miR-125b Expression in Possible Underlying Mechanisms. rbmb.net 2025; 14 (1) :145-154
URL: http://rbmb.net/article-1-1672-en.html
URL: http://rbmb.net/article-1-1672-en.html
Department of Medical Basic Sciences, Shoushtar Faculty of Medical Sciences, Shoushtar, Iran.
Abstract: (283 Views)
Background: This study examined the protective effects of zinc oxide nanoparticles (ZnO-NPs) on hepatic ischemia-reperfusion injury (HIRI) and their possible underlying mechanisms.
Methods: 48 male rats were randomly divided into six groups (n=8): the sham group that received intraperitoneal normal saline solution (Sham), the HIRI group, the control groups pre-treated with 5 and 10 mg/kg ZnO-NPs for 3 consecutive days without surgery (ZnO5) and (ZnO10), the HIRI group pre-treated with 5 mg/kg ZnO-NPs for 3 consecutive days before surgery (HIRI+ZnO5), and the HIRI group pre-treated with 10 mg/kg ZnO-NPs for 3 consecutive days before surgery (HIRI+ZnO10). One hour after reperfusion, serum and tissue samples were collected for biochemical, molecular and histopathological evaluation.
Results: Administration of ZnO-NPs caused significant improvement in the elevated serum concentrations of ALT, AST, TOS and MDA, improved liver histopathology, and increased TNF-α, IL-6, and NF-κB levels in liver tissue compared to HIRI group. In addition, administration of ZnO-NPs increased the expression of miR-125 in liver tissue compared than in the HIRI group.
Conclusion: The administration of ZnO-NPs improved the effect on HIRI by enhancing miR-125b expression and suppressing oxidative stress and inflammatory cytokines.
Methods: 48 male rats were randomly divided into six groups (n=8): the sham group that received intraperitoneal normal saline solution (Sham), the HIRI group, the control groups pre-treated with 5 and 10 mg/kg ZnO-NPs for 3 consecutive days without surgery (ZnO5) and (ZnO10), the HIRI group pre-treated with 5 mg/kg ZnO-NPs for 3 consecutive days before surgery (HIRI+ZnO5), and the HIRI group pre-treated with 10 mg/kg ZnO-NPs for 3 consecutive days before surgery (HIRI+ZnO10). One hour after reperfusion, serum and tissue samples were collected for biochemical, molecular and histopathological evaluation.
Results: Administration of ZnO-NPs caused significant improvement in the elevated serum concentrations of ALT, AST, TOS and MDA, improved liver histopathology, and increased TNF-α, IL-6, and NF-κB levels in liver tissue compared to HIRI group. In addition, administration of ZnO-NPs increased the expression of miR-125 in liver tissue compared than in the HIRI group.
Conclusion: The administration of ZnO-NPs improved the effect on HIRI by enhancing miR-125b expression and suppressing oxidative stress and inflammatory cytokines.
Type of Article: Original Article |
Subject:
Molecular Biology
Received: 2025/05/22 | Accepted: 2025/10/19 | Published: 2025/12/9
Received: 2025/05/22 | Accepted: 2025/10/19 | Published: 2025/12/9
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