Volume 7, Issue 2 (Vol.7 No.2 Jan 2019)                   rbmb.net 2019, 7(2): 119-128 | Back to browse issues page

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Mokhtariye A, Hagh-Nazari L, Varasteh A, Keyfi F. Diagnostic methods for Lysosomal Storage Disease. rbmb.net. 2019; 7 (2) :119-128
URL: http://rbmb.net/article-1-218-en.html
Department of Medical Laboratory Sciences, Varastegan Institute for Medical Sciences, Mashhad, Iran.
Abstract:   (1046 Views)
Lysosomal storage disorders (LSD) are a class of metabolic disturbance in which manifested by the accumulation of large molecules (complex lipids, glycoproteins, glycosaminoglycans, etc.) in lysosomes. LSDs have a wide range of clinical symptoms that may contain organ dysfunction, neurological and skeletal disorders. The first stage of diagnosis is clinically suspected by a physician. Next stage is enzyme activity assays including Fluorometry and MS/MS methods. These methods usually placed in newborn program screening. The second laboratory diagnostic stage is molecular examination (RFLP-PCR and ARMS-PCR, Mutations Scanning Methods, DNA sequencing, MLPA and NGS methods) that is confirmation of the enzyme assays. In this article, routine diagnostic methods for LSDs were discussed. The gold standard for enzyme activity assay and molecular diagnosis is TMS and NGS, respectively.
Full-Text [PDF 466 kb]   (1145 Downloads)    
Type of Article: Review | Subject: Biochemistry
Received: 2018/01/23 | Accepted: 2018/04/2 | Published: 2018/10/28

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