Volume 7, Issue 2 (Vol.7 No.2 Jan 2019)                   rbmb.net 2019, 7(2): 167-173 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Derakhshan A, Tavakkol Afshari J, Sadeghi Allah Abadi J, Nikpoor A R, Daneshvar R, Shokoohi Rad S et al . The Association Between the Transforming Growth Factor Beta-1 -509C>T Gene Polymorphism and Primary Open Angle Glaucoma in North Eastern Iran. rbmb.net 2019; 7 (2) :167-173
URL: http://rbmb.net/article-1-221-en.html
Eye research center, Khatam-Al-Anbia eye hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
Abstract:   (4258 Views)
Background: Glaucoma is a common cause of irreversible blindness. Transforming growth factor beta-1(TGF-β1) is the main isoform of TGF-β superfamily in the eye. Overexpression of TGF-β1 is shown to be related with the glaucoma. Studies have shown that the presence of mutant T allele of TGF-β1 -509C>T polymorphism (rs1800469) is associated with increased gene expression. So, in present study, association of the TGF-β1-509C>T gene polymorphism and primary open angle glaucoma (POAG) in patients from north east of Iran was investigated.

Methods: A case-control study was conducted on 112 POAG patients and 112 control participants. TGF-β1-509C>T genotyping was done by PCR-restriction fragment length polymorphism (PCR-RFLP) method using Bsu36I restriction enzyme. Moreover, cup to disk ratio(CDR), intraocular pressure (IOP) and visual acuity (VA) were measured. The obtained results were statistically analyzed.

Results: The highest frequency of genotype in the control group was related to CC genotype (44.6%), but the heterozygous CT genotype (45.6%) was observed as the highest frequency of genotypes in patient group (P value: 0.022, OR for TT genotype: 2.54 CI95% for OR: 1.22, 5.27). Also, the frequency of the T mutant allele showed a significant difference between case and control groups (P value: 0.005, OR: 1.73 CI95% for OR: 1.18, 2.53).  

Conclusions: In conclusion, a significant association was seen between TGF-β1 -509C>T gene polymorphism and POAG disease and inheritance of mutant T allele is considered to be a risk factor for glaucoma in patients living in North Eastern part of Iran.
 
Full-Text [PDF 524 kb]   (1918 Downloads)    
Type of Article: Original Article | Subject: Immunology
Received: 2018/01/27 | Accepted: 2018/04/29 | Published: 2018/10/28

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2015 All Rights Reserved | Reports of Biochemistry and Molecular Biology

Designed & Developed by : Yektaweb