Volume 1, Issue 2 (Vol.1 No.2 Apr 2013)                   rbmb.net 2013, 1(2): 69-73 | Back to browse issues page

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Saadabadi F, Mohajery M, Poostchi E, Shamsian S A A. Identification of Leishmania Species Causing Cutaneous Leishmaniasis Using Random Amplified Polymorphic DNA (RAPD-PCR) in Kharve, Iran. rbmb.net. 2013; 1 (2) :69-73
URL: http://rbmb.net/article-1-32-en.html
Parasitology & Medical Mycology Department, School of Medicine, Mashhad University of Medical Sciences Mashhad, Iran
Abstract:   (3116 Views)

Background: Leishmaniasis, especially cutaneous leishmaniasis, is considered an important health problem in many parts of Iran including Kharve, Khorasan Razavi province. Cutaneous leishmaniasis is caused by various species of Leishmania, each having a different secondary host. Thus, identifying the parasites’ specie is of paramount importance for containment strategy planning. The morphological differentiation of Leishmania species is not possible, rendering the molecular methods as the sole means to this purpose. Therefore, to identify the causative agent of cutaneous leishmaniasis in Kharve, Random Amplified Polymorphic DNA-PCR (RAPD-PCR) was used.

Methods: The disease was first confirmed by direct smears. Samples were gathered from 22 patients with established cutaneous leishmaniasis. The samples were immediately cultured in NNN medium, followed by sub-culture in RPMI-1640. Afterwards, DNA was extracted and amplified using RAPD-PCR. Electrophoresis patterns from each isolate were compared with reference strains of Leishmania major (L. major) and Leishmania tropica (L. tropica).

Results: The results of this study indicated that the parasite causing cutaneous leishmaniasis in Kharve is L. tropica.

Conclusion: It seems that L. tropica is the only causative agent of cutaneous leishmaniasis in Kharve, and RAPD-PCR is a suitable tool for Leishmania characterization in epidemiological studies.

Full-Text [PDF 390 kb]   (895 Downloads)    
Subject: Microbiology
Received: 2016/08/21 | Accepted: 2016/08/21 | Published: 2016/08/21

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