Reports of Biochemistry

Mendeley  

Zotero  

Rezai M, Saravani R, Sargazi S, Moudi M, Jafari Shahroudi M, Saravani R. Achillea Wilhelmsii C. KochHydroalcoholic Extract Induces Apoptosis and Alters LIN28B and p53 Gene Expression in Hela Cervical Cancer Cells. rbmb.net 2019; 8 (3) :318-325
URL: http://rbmb.net/article-1-340-en.html

Background:Inappropriate activation of the proto-oncogene LIN28Band inactivation of the p53 tumor suppressor, have been shown to have a critical role in tumorigenesis. Previousresearch has shown therapeutic potential for the use of herbal plants as an alternative strategyforcancer treatment. Achillae wilhelmsii C. Kochis a plant that has been traditionally used for its medicinal properties. The aim of this study was to investigate the cytotoxic and apoptosis-inducing effect of Achillea wilhelmsiiC. Kochhydroalcoholicextract (AWHE) on HeLa cervical cancer cells and its effect on LIN28Band p53 expression.

Methods:The cytotoxic activity of AWHEwas evaluated on HeLa cells using a trypan blue exclusion assay. The Annexin V/PI double staining assay was used to evaluate the apoptosis-inducing effect of the extract. The expressionof LIN28Band p53mRNA was measured using the real-time-PCRmethod.

Results:Treatment with AWHEwas shown to induce cytotoxicity in both time and concentration-dependent manners(P<0.05). The propositionof HeLa cells undergoing apoptosis increased with increasing concentrations of AWHE(P<0.05). The mRNA levels of p53increased following 12, 24, and 48 hours of AWHEtreatmentwhereasthe mRNA levels of LIN28Bwere significantly decreased after4 to 12 hours of AWHEtreatment (p<0.05).

Conclusions:Our findings confirmed the pro-apoptotic function of AWHEon the cervical cancer HeLa cell line. This indicates that targeting the LIN28Bsignaling cascade may be a promising therapeutic strategy for cervical cancer. Further research is required to understand the therapeutic effects of AWHEin primary human cervical cancer cells and a pre-clinicalcervical cancer model.