Volume 8, Issue 3 (Vol.8 No.3 Oct 2019)                   rbmb.net 2019, 8(3): 326-334 | Back to browse issues page

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Soleymani B, Parvaneh S, Mostafaie A. Goat Polyclonal Antibody Against the Sex Determining Region Y to Separate X- and Y-Chromosome Bearing Spermatozoa. rbmb.net. 2019; 8 (3) :326-334
URL: http://rbmb.net/article-1-360-en.html
Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Abstract:   (501 Views)
Background: Sex selection of sperm by separating X- and Y-chromosome bearing spermatozoa is critical for efficiently obtaining the desired sex of animal offspring in the livestock industry. The purpose of this study was to produce a goat polyclonal antibody (pAb) against the bovine Sex Determining Region Y
chromosome (bSRY) to separate female- and male-bearing spermatozoa.

Methods: To produce a goat polyclonal antibody against bSRY, a female goat was subcutaneously immunized with 27 kDa of recombinant bSRY (rbSRY) protein as the antigen. The anti-bSRY pAb was purified by ion-exchange chromatography. The purity of the pAb was determined using the SDS-PAGE
method. The biological activity of the anti-bSRY pAb was examined using PCR to assess the binding affinity of pAb for the bSRY antigen and commercially sexed bull sperm.

Results: The total amount of purified anti-bSRY pAb was approximately 650 mg/goat serum (13 mg/mL). Interestingly, our data showed that the binding affinity of our pAb to the Y bearing was high, while the binding affinity of that to the X-chromosome bearing sperm was similar to the negative control.

Conclusions: In conclusion, our findings show that the goat anti-SRY pAb specifically binds to Ychromosome bearing sperm that suggesting its potential use for sex selection.
Full-Text [PDF 450 kb]   (224 Downloads)    
Type of Article: Original Article | Subject: Immunology
Received: 2019/05/25 | Accepted: 2019/12/15 | Published: 2020/01/24

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