Volume 2, Issue 1 (Vol.2 No.1 Oct 2013)                   rbmb.net 2013, 2(1): 35-41 | Back to browse issues page

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Torabi A, Tahmoorespour M, Vahedi F, Mosavari N, Nassiri M. Construction of Eukaryotic Expression Vectors Encoding CFP-10 and ESAT-6 Genes and Their Potential in Lymphocyte Proliferation. rbmb.net. 2013; 2 (1) :35-41
URL: http://rbmb.net/article-1-39-en.html
Razi Vaccine and Serum Research Institute, Mashhad, Iran
Abstract:   (4926 Views)

Background: Mycobacterium (M.) bovis is the agent of bovine tuberculosis (TB) in a range of animal species, including humans. Recent advances in immunology and the molecular biology of Mycobacterium have allowed identification of a large number of antigens with the potential for the development of a new TB vaccine. The ESAT-6 and CFP-10 proteins of M. bovis are important structural and functional proteins known to be important immunogens.

Methods: In the current study, the DNAs encoding these genes were utilized in the construction of pcDNA 3.1+/ESAT-6 and pcDNA3.1+/CFP-10 plasmids. After intramuscular injection of BALB/c mice with these plasmids, ESAT-6 and CFP-10 mRNA expression was assessed by RT-PCR. Mice were inoculated and boosted with the plasmids to evaluate their effects on lymphocyte proliferation.

Results: Our results indicate the plasmids are expressed at the RNA level and can induce lymphocyte proliferation.

Conclusion: Further study is needed to characterize the effect of these antigens on the immune system and determine whether they are effective vaccine candidates against M. bovis.

Full-Text [PDF 243 kb]   (1277 Downloads)    
Subject: Microbiology
Received: 2013/07/12 | Accepted: 2013/08/10 | Published: 2013/10/30

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