Volume 8, Issue 4 (Vol.8 No.4 Jan 2020)                   rbmb.net 2020, 8(4): 419-428 | Back to browse issues page

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Reza H M, Saleh R, Jain P, Rahman G M S, Bepari A K. C-MAF Expression in Adult Human Ocular Surface and its Implication in Pterygium Pathogenesis. rbmb.net 2020; 8 (4) :419-428
URL: http://rbmb.net/article-1-417-en.html
Department of Pharmaceutical Sciences, North South University, Dhaka 1229, Bangladesh.
Abstract:   (3049 Views)
Background: c-MAF, a transcription factor that belongs to the b-Zip Maf transcription factor family, was found to be critical for lens development in vertebrates. It is a well-known fact that the adult human ocular surface expresses c-MAF, however, its role in the limbus, cornea and conjunctiva remains unknown. Thus, the present study aimed to investigate c-MAF expression within the human ocular surface, and its potential role in pterygium pathogenesis.

Methods: We performed immunohistochemical staining to detect c-MAF expression in frozen adult human tissue sections, including the limbus, cornea and conjunctiva, and cultured cells from eye cadavers. We then compared c-MAF expression to the expression of a known protein, P63. Lastly, we performed RT-PCR, and immunohistochemistry for c-MAF expression in healthy adult human conjunctiva and pterygium.

Results: We found differential c-MAF expression between adult human limbus, cornea and conjunctiva tissues. Further, we observed that c-MAF is downregulated in the pterygium compared to healthy conjunctiva.

Conclusions: Overall, our results suggest that c-MAF may play a context-specific role in maintaining limbal, corneal and conjunctival homeostasis, and may be critical for preventing pterygium development in humans.
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Type of Article: Original Article | Subject: Cell Biology
Received: 2019/11/5 | Accepted: 2019/12/22 | Published: 2020/05/10

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